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master's thesis
Adhezijska svojstva bakterija mliječne kiseline izoliranih iz majčinog mlijeka
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:309711
Hrsan, Jana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Hrsan, J. (2023). Adhezijska svojstva bakterija mliječne kiseline izoliranih iz majčinog mlijeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:309711

Hrsan, Jana. "Adhezijska svojstva bakterija mliječne kiseline izoliranih iz majčinog mlijeka." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:309711

Hrsan, Jana. "Adhezijska svojstva bakterija mliječne kiseline izoliranih iz majčinog mlijeka." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:309711

Hrsan, J. (2023). 'Adhezijska svojstva bakterija mliječne kiseline izoliranih iz majčinog mlijeka', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 29 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:309711

Hrsan J. Adhezijska svojstva bakterija mliječne kiseline izoliranih iz majčinog mlijeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 29] Available at: https://urn.nsk.hr/urn:nbn:hr:159:309711

J. Hrsan, "Adhezijska svojstva bakterija mliječne kiseline izoliranih iz majčinog mlijeka", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:309711

Metadata
TitleAdhezijska svojstva bakterija mliječne kiseline izoliranih iz majčinog mlijeka
Title (english)Adhesion properties of lactic acid bacteria isolated from human breast milk
AuthorJana Hrsan
MentorAndreja Leboš Pavunc (mentor)
MentorKrešo Bendelja (komentor)
MentorNina Čuljak (pomoć pri izradi, neposredni voditelj)
MentorŽeljko Cvetić (pomoć pri izradi, neposredni voditelj)
Committee memberBlaženka Kos (predsjednik povjerenstva)
Committee memberMarina Cvjetko Bubalo (član povjerenstva)
Committee memberKsenija Durgo (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2023-07-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada bio je odrediti mogu li se sojevi Levilactobacillus brevis MB1, MB2, MB13, MB20 i Limosilactobacillus fermentum MC1, izolirani iz majčinog mlijeka, potencijalno koristiti kao probiotici. Ispitana je uloga S-proteina i egzopolisaharida bakterijskih sojeva u adheziji i kompetitivnoj ekskluziji bakterije Salmonella enterica serovar Typhimurium FP1, primjenom Caco-2 stanične linije. Adhezijska sposobnost istih sojeva također je potvrđena ispitivanjem vezanja sojeva na lektinu sličan protein ZG16. Ispitivanjem imunomodulacijskog učinka izoliranih S-proteina i egzopolisaharida te sojeva producenata, korištenjem Caco-2 stanične linije tretirane s lipopolisaharidima porijeklom iz bakterije Escherichia coli i hTNF-α, pokazano je njihovo inhibitorno djelovanje na proizvodnju proupalnih citokina IL-6, IL-8, IL-1β i TNF-α.
Abstract (english)
The aim of this work was to determine whether strains of Levilactobacillus brevis MB1, MB2, MB13, MB20 and Limosilactobacillus fermentum MC1, isolated from breast milk, could be used as potential probiotics. The role of S-proteins and exopolysaccharides of bacterial strains on intestinal epithelial Caco-2 cell line adhesion and competitive exclusion of the bacterium Salmonella enterica serovar Typhimurium FP1 was tested. The adhesion ability of the same strains was also confirmed by examining the binding of the strains to the lectin-like protein ZG16. The immunomodulatory effect of strain specific isolated S-proteins or exopolysaccharides and live bacteria using Caco-2 cells treated with lipopolysaccharides from Escherichia coli and human TNF-α was assesed. Inhibition of proinflammatory cytokines IL-6, IL-8, IL-1β and TNF-α production was demonstrated.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:309711
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-06-21 09:05:31