prikaz prve stranice dokumenta Optimization of O-demethylation of low molecular weight compounds from lignin fractions
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master's thesis
Optimization of O-demethylation of low molecular weight compounds from lignin fractions
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:708168
Božić, Lidija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Božić, L. (2023). Optimization of O-demethylation of low molecular weight compounds from lignin fractions (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:708168

Božić, Lidija. "Optimization of O-demethylation of low molecular weight compounds from lignin fractions." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:708168

Božić, Lidija. "Optimization of O-demethylation of low molecular weight compounds from lignin fractions." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:708168

Božić, L. (2023). 'Optimization of O-demethylation of low molecular weight compounds from lignin fractions', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 29 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:708168

Božić L. Optimization of O-demethylation of low molecular weight compounds from lignin fractions [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 29] Available at: https://urn.nsk.hr/urn:nbn:hr:159:708168

L. Božić, "Optimization of O-demethylation of low molecular weight compounds from lignin fractions", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:708168

Metadata
TitleOptimization of O-demethylation of low molecular weight compounds from lignin fractions
Title (croatian)Optimiranje O-demetilacije niskomolekulskih spojeva nastalih frakcioniranjem lignina
AuthorLidija Božić
MentorAnita Slavica (mentor)
Committee memberRenata Teparic (predsjednik povjerenstva)
Committee memberBlaženka Kos (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2023-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Optimal O-demethylation of guaiacol to catechol by unspecific peroxygenase 12 (UPO12) were obtained at 20 °C and pH of 6.0. The reaction follows Michaelis-Menten kinetics and Vₘₐₓ and Kₘ were determined (106.87 µmol/(L*min) and 8.29 µmol/(L*min), respectively). The initial co-substrate concentration higher than 10 mM reduces initial rate of the guaiacol consumption. Additional six UPO enzymes were considered for possible industrially relevant O-demethylation in batch and fed batch mode, and the best catechol producers were UPO12, UPO22, and UPO67. Immobilization success of UPO12 was lower than 50.0 %. Disproportional utilization of guaiacol and production of catechol occurs due to oxidation and dimerization of the substrate. The most encouraging fed batch conversions were obtained with initial concentration of the UPO12 of 0.05 µM, the guaiacol and H₂O₂ of 0.6 mM and with addition of all three components of the reaction mixture every 60 min in the same concentration over 6 h.
Abstract (croatian)
Optimalna O-demetilacija gvajakola u katehol pomoću nespecifične peroksigenaze 12 (UPO12) provedena je pri 20 °C i pH 6.0. Ova reakcija prati Michaelis-Menten kinetiku i određeni su Vₘₐₓ i Kₘ (redom, 106,87 µmol/(L*min) i 8,29 µmol/(L*min)). Početna koncentracija ko-supstrata veća od 10 mM reducira početnu brzinu potrošnje gvajakola. Dodatnih šest UPO enzima istraženo je za industrijski relevantnu O-demetilaciju supstrata batch i fed batch postupkom i najbolji proizvođači katehola su bili UPO12, UPO22, i UPO67. Uspješnost imobilizacije UPO12 bila je manja od 50.0 %. Neproporcionalna potrošnja gvajakola i proizvodnja katehola javlja se zbog oksidacije i dimerizacije ovog supstrata. Najučinkovitija fed batch konverzija postignuta je uz početnu koncentraciju UPO12 od 0,05 µM, gvajakola i H₂O₂ od 0,6 mM i uz dodatak svih triju komponenti reakcijske smjese svakih 60 min u istovjetnim koncentracijama kroz 6 h.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:159:708168
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsClosed access
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Created on2024-06-24 12:13:18