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master's thesis
In vitro učinci resveratrola i ferocenskog derivata resveratrola na kulture stanica ovarija i keratinocita
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:525338
Doskočil, Bruno
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Toxicology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Doskočil, B. (2023). In vitro učinci resveratrola i ferocenskog derivata resveratrola na kulture stanica ovarija i keratinocita (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:525338

Doskočil, Bruno. "In vitro učinci resveratrola i ferocenskog derivata resveratrola na kulture stanica ovarija i keratinocita." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:525338

Doskočil, Bruno. "In vitro učinci resveratrola i ferocenskog derivata resveratrola na kulture stanica ovarija i keratinocita." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:525338

Doskočil, B. (2023). 'In vitro učinci resveratrola i ferocenskog derivata resveratrola na kulture stanica ovarija i keratinocita', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:525338

Doskočil B. In vitro učinci resveratrola i ferocenskog derivata resveratrola na kulture stanica ovarija i keratinocita [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:525338

B. Doskočil, "In vitro učinci resveratrola i ferocenskog derivata resveratrola na kulture stanica ovarija i keratinocita", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:525338

Metadata
TitleIn vitro učinci resveratrola i ferocenskog derivata resveratrola na kulture stanica ovarija i keratinocita
Title (english)In vitro effects of resveratrol and ferrocene derivative of resveratrol on ovarian and keratinocyte cell cultures
AuthorBruno Doskočil
MentorIvana Kmetič (mentor)
MentorMarina Miletić (pomoć pri izradi, neposredni voditelj)
MentorTeuta Murati (pomoć pri izradi, neposredni voditelj)
Committee memberTeuta Murati (predsjednik povjerenstva)
Committee memberKristina Radošević (član povjerenstva)
Committee memberIgor Slivac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Toxicology)
Zagreb
Defense date and country2023-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom radu ispitan je učinak resveratrola (RSV) te ferocenskog derivata resveratrola (RF) (5, 20, 50 i 100 µM) na indukciju stanične smrti u kulturama stanica ovarija (CHO-K1 stanična linija) i keratinocita (HaCaT stanična linija). Uz to, praćena je i vijabilnost te proliferacija stanica metodama Neutral Red i Trypan Blue. Nakon tretmana stanica RSV-om u obje stanične linije zabilježene su niže IC vrijednosti dobivene Trypan Blue metodom u odnosu na Neutral Red test što se može objasniti povećanjem broja i veličine lizosoma te većom količinom vezane boje Neutral Red u tretiranih stanica u odnosu na netretirane. Tretman HaCaT stanica RSV-om rezultirao je blago povećanim udjelom stanica u apoptozi u odnosu na kontrolni uzorak, dok je u CHO-K1 kulturi stanica taj učinak bio izraženiji i statistički značajan (u dozama ≥20 μM ). RSV je potaknuo autofagiju u obje kulture stanica, a vrijednost autophagy induction ratio varira ovisno o primijenjenoj koncentraciji. RF je pri dozama ≥ 20 µM uzrokovao statistički značajno (p< 0,001) smanjenje udjela stanica koje odumiru autofagijom u odnosu na kontrolni uzorak, što ukazuje na izmijenjenu biološku aktivnost od djelovanja samog RSV-a koji statistički značajno inducira autofagiju u CHO-K1 kulturi stanica.
Abstract (english)
In this study, the effect of resveratrol (RSV) and the ferrocene derivative of resveratrol (RF) (5, 20, 50 and 100 µM) on the induction of cell death in ovarian cell culture (CHO-K1 cell line) and keratinocytes (HaCaT cell line) was determined. In addition, viability and cell proliferation were monitored using the Neutral Red and Trypan Blue methods. After treatment of cells with RSV, lower IC values obtained by the Trypan Blue method compared to the Neutral Red test were recorded in both cell lines, which can be explained by an increase in the number and size of lysosomes. Treatment of HaCaT cells with RSV resulted in a slightly increased proportion of cells undergoing apoptosis compared to the control sample, whereas in CHO-K1 cell culture this effect was more pronounced and statistically significant (at doses ≥20 μM). RSV induced autophagy in both cell cultures, and the value of the autophagy induction ratio varied depending on the concentration applied. At doses ≥ 20 µM, RF caused a statistically significant (p< 0.001) decrease in the proportion of cells that die by autophagy compared to the control sample, indicating altered biological activity of RF compared to the action of RSV itself (which induced autophagy in CHO-K1 cell culture).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:525338
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-06-27 09:44:46