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master's thesis
Mikrovalovima potpomognuta ekstrakcija bioaktivnih spojeva iz odabranih morskih algi i spužve
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:196651
Špoljar, Željka
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for drying Technologies and monitoring of biologically active compounds

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Špoljar, Ž. (2023). Mikrovalovima potpomognuta ekstrakcija bioaktivnih spojeva iz odabranih morskih algi i spužve (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:196651

Špoljar, Željka. "Mikrovalovima potpomognuta ekstrakcija bioaktivnih spojeva iz odabranih morskih algi i spužve." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:196651

Špoljar, Željka. "Mikrovalovima potpomognuta ekstrakcija bioaktivnih spojeva iz odabranih morskih algi i spužve." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:196651

Špoljar, Ž. (2023). 'Mikrovalovima potpomognuta ekstrakcija bioaktivnih spojeva iz odabranih morskih algi i spužve', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:196651

Špoljar Ž. Mikrovalovima potpomognuta ekstrakcija bioaktivnih spojeva iz odabranih morskih algi i spužve [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:196651

Ž. Špoljar, "Mikrovalovima potpomognuta ekstrakcija bioaktivnih spojeva iz odabranih morskih algi i spužve", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:196651

Metadata
TitleMikrovalovima potpomognuta ekstrakcija bioaktivnih spojeva iz odabranih morskih algi i spužve
Title (english)Microwave-assisted extraction of bioactive compounds from selected marine algae and sponge
AuthorŽeljka Špoljar
MentorSandra Pedisić (mentor)
MentorZrinka Čošić (pomoć pri izradi, neposredni voditelj)
Committee memberMaja Repajić (predsjednik povjerenstva)
Committee memberIvona Elez Garofulić (član povjerenstva)
Committee memberVerica Dragović-Uzelac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Engineering)
(Laboratory for drying Technologies and monitoring of biologically active compounds)
Zagreb
Defense date and country2023-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Cilj ovog rada bio je utvrditi maseni udio ukupnih fenola i pigmenata u ekstraktima morske spužve i raznih vrsta algi dobivenih ekstrakcijom uz pomoć mikrovalova. Više uzoraka algi vrsta Fucus virsoides i Cystoseira barbata uzorkovano je u različito vrijeme. Ukupni fenoli određeni su spektrofotometrijski u analiziranim uzorcima u rasponu od 34,6 do 3607,8 mg/100 g s.tv. HPLC metodom određeni su i ukupni karotenoidi (13,83-770,95 mg/100 g s.tv.) i klorofili (1,34-292,01 mg/100 g s.tv.). Antioksidacijska aktivnost određena je ORAC (119,33-411,72 µmol Trolox/100 g s.tv.), ABTS (96,40-6473,86 µmol Trolox/100 g s.tv.) i DPPH metodom (1733,38-3200,85 µmol Trolox/100 g s.tv.). Utvrđene su statistički značajne razlike u masenim udjelima ukupnih fenola, karotenoida i klorofila u ispitivanim uzorcima. Maseni udio ukupnih fenola u smeđim algama Fucus virsoides i Cystoseira barbata varirao je ovisno o godišnjem dobu i bio je najveći u jesen.
Abstract (english)
The aim of this study was to determine the mass fraction of total phenols and pigments in extracts of marine sponge and various algal species obtained by microwave-assisted extraction. Several samples of algae species Fucus virsoides and Cystoseira barbata were sampled at different times. Total phenols were determined spectrophotometrically in the analyzed samples and ranged from 34.6 to 3607.8 mg/100 g dm. Total carotenoids (13.83-770.95 mg/100 g dm) and chlorophylls (1.34-292.01 mg/100 g dm) were also determined by the HPLC method. Antioxidant activity was determined by ORAC (119.33- 411.72 µmol Trolox/100 g dm), ABTS (96.40-6473.86 µmol Trolox/100 g dm) and DPPH method (1733.38-3200.85 µmol Trolox/100 g dm). Statistically significant differences were found in the mass fractions of total phenolics, carotenoids and chlorophyll in the studied samples. The mass fraction of total phenolics in the brown algae Fucus virsoides and Cystoseira barbata varied depending on the season and was highest in autumn.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:196651
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) icon
Created on2024-07-03 10:16:43