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master's thesis
Biološka aktivnost ferocenskih konjugata s homo- i heterokiralnim Ala-Pro dipeptidima određena na HeLa staničnoj liniji
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:901822
Ivić, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Ivić, P. (2024). Biološka aktivnost ferocenskih konjugata s homo- i heterokiralnim Ala-Pro dipeptidima određena na HeLa staničnoj liniji (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:901822

Ivić, Petra. "Biološka aktivnost ferocenskih konjugata s homo- i heterokiralnim Ala-Pro dipeptidima određena na HeLa staničnoj liniji." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:901822

Ivić, Petra. "Biološka aktivnost ferocenskih konjugata s homo- i heterokiralnim Ala-Pro dipeptidima određena na HeLa staničnoj liniji." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:901822

Ivić, P. (2024). 'Biološka aktivnost ferocenskih konjugata s homo- i heterokiralnim Ala-Pro dipeptidima određena na HeLa staničnoj liniji', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:901822

Ivić P. Biološka aktivnost ferocenskih konjugata s homo- i heterokiralnim Ala-Pro dipeptidima određena na HeLa staničnoj liniji [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:901822

P. Ivić, "Biološka aktivnost ferocenskih konjugata s homo- i heterokiralnim Ala-Pro dipeptidima određena na HeLa staničnoj liniji", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:901822

Metadata
TitleBiološka aktivnost ferocenskih konjugata s homo- i heterokiralnim Ala-Pro dipeptidima određena na HeLa staničnoj liniji
Title (english)Biological activity of ferrocene conjugates with homo- and heterochiral Ala Pro dipeptides determined on HeLa cell line
AuthorPetra Ivić
MentorKristina Radošević (mentor)
MentorMartina Bagović (pomoć pri izradi, neposredni voditelj)
Committee memberMonika Kovačević (predsjednik povjerenstva)
Committee memberVišnja Gaurina Srček (član povjerenstva)
Committee memberLidija Barišić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2024-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Konjugacijom organometalnog spoja ferocena s biomolekulama nastaju kemijski raznoliki spojevi koje odlikuje iznimna stabilnost, netoksičnost i povećana bioaktivnost. U ovom je radu ispitana in vitro biološka aktivnost osam ferocenskih konjugata s homo- i heterokiralnim dipeptidom pripravljenim iz alanina (Ala) i prolina (Pro) na humanoj tumorskoj staničnoj liniji HeLa. Antitumorski učinak je ispitan određivanjem stanične vijabilnosti te klonogenom analizom. Protočnom citometrijom određeno je dolazi li do aktivacije procesa stanične smrti. Ispitivani ferocenski peptidomimetici imaju inhibitorni učinak na rast HeLa stanične linije. Najjače citotoksično djelovanje pokazuju spojevi 1, 2 i 5. Inhibitorno djelovanje spojeva 1 i 2 može se povezati s poticanjem stanične smrti apoptozom. Najizraženiji antitumorski potencijal pokazuju spojevi koji sadrže tert-butoksikarbonilnu (Boc)-zaštitnu skupinu te L-Pro, odnosno spojevi 1 i 2.
Abstract (english)
By conjugating the organometallic compound ferrocene with biomolecules, chemically diverse compounds are formed, characterized by exceptional stability, non-toxicity, and increased bioactivity. This study examined the in vitro biological activity of eight ferrocene conjugates with homo- and heterochiral dipeptide formed of alanine (Ala) and proline (Pro) on the human tumor cell line HeLa. The antitumor effect was examined by determining cell viability and performing a clonogenic assay. Flow cytometry was used to determine whether the process of cell death is activated. The investigated ferrocene peptidomimetics have an inhibitory effect on the growth of the HeLa cell line. Compounds 1, 2, and 5 show the strongest cytotoxic activity. The inhibitory effect of compounds 1 and 2 can be linked to the induction of cell death by apoptosis. The compounds containing the tert-butoxycarbonyl (Boc)-protective group and L-Pro, specifically compounds 1 and 2, show the most pronounced antitumor potential.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:901822
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-08-29 08:27:42