prikaz prve stranice dokumenta Protein Taf14 je uključen u remodeliranje strukture kromatina promotora pri aktivaciji transkripcije gena PHO5 u kvascu
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undergraduate thesis
Protein Taf14 je uključen u remodeliranje strukture kromatina promotora pri aktivaciji transkripcije gena PHO5 u kvascu
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:239395
Kordić, Stela
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kordić, S. (2014). Protein Taf14 je uključen u remodeliranje strukture kromatina promotora pri aktivaciji transkripcije gena PHO5 u kvascu (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:239395

Kordić, Stela. "Protein Taf14 je uključen u remodeliranje strukture kromatina promotora pri aktivaciji transkripcije gena PHO5 u kvascu." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:239395

Kordić, Stela. "Protein Taf14 je uključen u remodeliranje strukture kromatina promotora pri aktivaciji transkripcije gena PHO5 u kvascu." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:239395

Kordić, S. (2014). 'Protein Taf14 je uključen u remodeliranje strukture kromatina promotora pri aktivaciji transkripcije gena PHO5 u kvascu', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:239395

Kordić S. Protein Taf14 je uključen u remodeliranje strukture kromatina promotora pri aktivaciji transkripcije gena PHO5 u kvascu [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:159:239395

S. Kordić, "Protein Taf14 je uključen u remodeliranje strukture kromatina promotora pri aktivaciji transkripcije gena PHO5 u kvascu", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:239395

Metadata
TitleProtein Taf14 je uključen u remodeliranje strukture kromatina promotora pri aktivaciji transkripcije gena PHO5 u kvascu
Title (english)Protein Taf14 is involved in promoter chromatin remodeling process during activation of yeast PHO5 gene trancription
AuthorStela Kordić
MentorSlobodan Barbarić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2014-07-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
U eukariotskim stanicama DNA se nalazi u kompleksu sa histonima, odnosno u strukturi kromatina, te je transkripcijskim faktorima koji sudjeluju u regulaciji transkripcije gena, onemogućeno vezanje na DNA. Dakle, osim strukturne uloge u kondenzaciji DNA, struktura kromatina djeluje represivno na proces transkripcije gena. Stoga su modifikacije kromatina i remodeliranje strukture kromatina promotorskih regija preduvjet za aktivaciju transkripcije. Protein Taf14 je podjedinica takva tri kompleksa SWI/SNF, Ino80 i RSC, koji sudjeluju u procesu remodeliranja strukture kromatina promotora gena PHO5. Također, Taf14 je i podjedinica općih transkripcijskih faktora TFIID i TFIIF, histon-acetiltransferaze NuA3 i globalnog ko-aktivatora transkripcije, Mediatora. Uloga proteina Taf14 u procesu remodeliranja strukture kromatina kao i regulaciji transkripcije gena predmet je iscrpnih istraživanja, ali do sada se o njegovoj specifičnoj ulozi u ovim procesima vrlo malo zna. U ovom radu je, korištenjem mutanta taf14, ispitan efekt inaktivacije ovog proteina na proces aktivacije promotora PHO5. Utvrđeno je da u odsutstvu proteina Taf14 dolazi do usporenog remodeliranja kromatina promotora PHO5 i posljedično usporene aktivacije transkripcije gena.
Abstract (english)
In eukaryotic cells, DNA exists as a complex with histone proteins, called chromatin, so that binding of transcription factors, which participate in transcriptional regulation, on DNA is disabled. Therefore, apart from its structural role in DNA condensation, chromatin structure acts repressive on gene transcription and chromatin remodeling is a prerequisite for promoter activation. Protein Taf14 is a subunit of three ATP- dependent remodeling complexes, SWI/SNF, Ino80 and RSC, involved in chromatin structure transition at the PHO5 promoter. It is also a subunit of general transcription factors TFIID and TFIIF, histon-acetyltransferase complex NuA3 and global co-activator of transcription, Mediator. The role of this protein as a subunit of many important proteins involved in chromatin remodeling and regulation of transcription is still mostly unknown. This work has examined the activation of PHO5 promoter in the absence of Taf14, using taf14 mutant cells. The results show a delayed PHO5 promoter chromatin remodeling process in absence of Taf14 and consequentially, a delayed activation of transcription.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:239395
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 13:09:18