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undergraduate thesis
Procjena protokola za pripremu uzoraka krvne plazme za određivanje butirata GC/MS metodom
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:250786
Krpan, Tara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Section for Bioinformatics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Krpan, T. (2024). Procjena protokola za pripremu uzoraka krvne plazme za određivanje butirata GC/MS metodom (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:250786

Krpan, Tara. "Procjena protokola za pripremu uzoraka krvne plazme za određivanje butirata GC/MS metodom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:250786

Krpan, Tara. "Procjena protokola za pripremu uzoraka krvne plazme za određivanje butirata GC/MS metodom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:250786

Krpan, T. (2024). 'Procjena protokola za pripremu uzoraka krvne plazme za određivanje butirata GC/MS metodom', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 14 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:250786

Krpan T. Procjena protokola za pripremu uzoraka krvne plazme za određivanje butirata GC/MS metodom [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2025 January 14] Available at: https://urn.nsk.hr/urn:nbn:hr:159:250786

T. Krpan, "Procjena protokola za pripremu uzoraka krvne plazme za određivanje butirata GC/MS metodom", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:250786

Metadata
TitleProcjena protokola za pripremu uzoraka krvne plazme za određivanje butirata GC/MS metodom
Title (english)Evaluation of the protocols for the preparation of bllod plasma samples for determination of butyrate by the GC/MS method
AuthorTara Krpan
MentorJurica Žučko (mentor)
MentorAndrijana Meščić Macan (pomoć pri izradi, neposredni voditelj)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Section for Bioinformatics)
Zagreb
Defense date and country2024-09-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom radu ispitivala se učinkovitost pripreme uzoraka krvne plazme za određivanje koncentracije butirata prema dva protokola. Koncentracija butirata indikator je zdravlja organizma, a butirat je važan zbog mnogih pozitivnih učinaka koje ima na ljudski organizam. Provedena su dva protokola pripreme uzorka i na kraju se uspoređivala njihova pogodnost za daljnju analizu na uređaju za GC/MS. Kao re zultat dobiveno je da su oba protokola dala zadovoljavajuće odvajanje faza i ekstrakciju kratkolanča nih masnih kiselina, ali za daljnju analizu ostalih uzoraka koristio bi se Protokol 1 zbog preciznijeg od vajanja faza i manje mogućnosti kontaminacije talogom.
Abstract (english)
In this study, we compared the efficiency of two protocols for preparing blood plasma samples for bu tyrate concentration determination using gas chromatography-mass spectrometry (GC/MS). Butyrate is a crucial biomarker of health due to its numerous beneficial effects on the human body. Both proto cols achieved satisfactory phase separation and extraction of short-chain fatty acids. However, Proto col 1 exhibited superior performance, demonstrating more precise phase separation and a reduced risk of sediment contamination. Therefore, Protocol 1 is recommended for future sample preparation in this context to ensure accurate and reliable butyrate concentration measurements using GC/MS. Keywords: butyric acid, GC/MS analysis, phase separation
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:250786
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-09-25 10:11:23