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undergraduate thesis
Fluorescentna in situ hibridizacija
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:008166
Krželj, Slavica
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Krželj, S. (2024). Fluorescentna in situ hibridizacija (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:008166

Krželj, Slavica. "Fluorescentna in situ hibridizacija." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:008166

Krželj, Slavica. "Fluorescentna in situ hibridizacija." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:008166

Krželj, S. (2024). 'Fluorescentna in situ hibridizacija', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:008166

Krželj S. Fluorescentna in situ hibridizacija [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:008166

S. Krželj, "Fluorescentna in situ hibridizacija", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:008166

Metadata
TitleFluorescentna in situ hibridizacija
Title (english)Fluorescent in situ hybridization
AuthorSlavica Krželj
MentorMarina Svetec Miklenić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2024-09-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Fluorescentna in situ hibridizacija (eng. fluorescent in situ hybridization, FISH) je učinkovita vizualna tehnika koja omogućuje identifikaciju i lokalizaciju određenih sekvenci DNA na kromosomima. Ovaj teorijski završni rad bavi se tehnikom FISH, njezinim povijesnim razvojem, primjenom u molekularnoj genetici i genetičkom inženjerstvu, te mogućnostima i ograničenjima pojedinih varijanti ove metode. Rad započinje kratkim povijesnim pregledom razvoja metoda za vizualizacije kromosoma i citogenetičkih postupaka, te razvojem samog FISH-a. Nadalje, u ovom radu opisane su različite varijacije ove metode, uključujući tzv. Multipleks FISH (eng. Multiplex Fluorescent in situ Hybridization, M-FISH) Fibre-FISH i interfazni FISH, te i njihove prednosti i ograničenja. Opisane su i kliničke primjene FISH-a, prvenstveno u dijagnostici nasljednih bolesti poput kronične mijeloične leukemije (eng. Chronic Myeloid Leukemia, CML) te razmatrane mogućnosti daljnjeg napretka u FISH tehnologiji.
Abstract (english)
Fluorescent in situ hybridization (FISH) is an effective visual technique that enables the identification and localization of specific DNA sequences on chromosomes. This theoretical thesis focuses on the FISH technique, its historical development, application in molecular genetics and genetic engineering, and the possibilities and limitations of certain variants of this method. The thesis first brings a brief historical overview of the development of methods for chromosome visualization and cytogenetic procedures, as well as the development of FISH itself. Furthermore, this paper describes various variations of this method, including the so-called Multiplex FISH (eng. Multiplex Fluorescent in situ Hybridization, M-FISH) Fibre-FISH and interphase FISH, and their advantages and limitations. Clinical applications of FISH are also described, primarily in the diagnosis of hereditary diseases such as Chronic Myeloid Leukemia (CML), and possibilities for further progress in FISH technology are discussed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:008166
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-09-26 08:27:05