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undergraduate thesis
Utjecaj prekomjerne sinteze proteina stanične stijenke Scw4p na fenotip kvasca S. cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:311458
Miškić, Terezija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Miškić, T. (2014). Utjecaj prekomjerne sinteze proteina stanične stijenke Scw4p na fenotip kvasca S. cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:311458

Miškić, Terezija. "Utjecaj prekomjerne sinteze proteina stanične stijenke Scw4p na fenotip kvasca S. cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:311458

Miškić, Terezija. "Utjecaj prekomjerne sinteze proteina stanične stijenke Scw4p na fenotip kvasca S. cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:311458

Miškić, T. (2014). 'Utjecaj prekomjerne sinteze proteina stanične stijenke Scw4p na fenotip kvasca S. cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:311458

Miškić T. Utjecaj prekomjerne sinteze proteina stanične stijenke Scw4p na fenotip kvasca S. cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:311458

T. Miškić, "Utjecaj prekomjerne sinteze proteina stanične stijenke Scw4p na fenotip kvasca S. cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:311458

Metadata
TitleUtjecaj prekomjerne sinteze proteina stanične stijenke Scw4p na fenotip kvasca S. cerevisiae
Title (english)The effect of over-expression of cell wall Scw4p protein on S. cerevisiae phenotype characteristics
AuthorTerezija Miškić
MentorRenata Teparić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2014-06-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Protein Scw4p je jedan od najzastupljenijih nekovalentno vezanih proteina stanične stijenke, čija fiziološka uloga u stijenci još uvijek nije poznata. U ovom radu je ispitivana potencijalna fiziološka uloga Scw4p ispitivanjem fenotipskih karakteristika mutiranih stanica kvasaca koje sintetiziraju povećanu količinu tog proteina. Prekomjerna sinteza Scw4p u stanicama divljeg tipa kvasca, kex2 mutanta i japsinskog mutanta dovodi do smanjenja viabilnosti stanica sva tri soja kvasca, pri čemu je veće smanjenje viabilnosti postignuto pri pH=4 nego pri pH=7. Prekomjerna sinteza Scw4p u stanicama divljeg tipa kvasca i japsinskog mutanta dovodi do povećanja osjetljivosti na djelovanje zimolijaze, dok kod stanica kex2 mutanta nema efekta. Na osnovu ovih rezultata može se zaključiti da protein Scw4p djeluje kao glukanaza, jer pri njegovoj povećanoj sintezi dolazi do pada vijabilnosti i povećanja osjetljivosti stanica na djelovanje hidrolitičkih enzima iz okoline. Enzimski aktivna je procesirana forma Scw4p, pri čemu se jači efekt aktivacije postiže djelovanjem Kex2p proteaze, a slabiji djelovanjem japsinskih proteaza.
Abstract (english)
One of the most abundant noncovalently linked cell wall proteins is Scw4p and its role in the cell wall has not yet been discovered. This paper examines the potential physiological role of this protein. The phenotype characteristics of mutant yeast cells that over-express Scw4p protein are questioned. The over-expression of Scw4p in wild type yeast cells, in kex2 mutant cells, and in yapsin mutants lowers the viability of every type of yeast cell. Greater infuluence of decreasing viability was achieved under pH=4 than under pH=7 conditions. Also, the over-expression of Scw4p in wild type yeast cells and in yapsin mutants increases the sensibility to zymoliase treatment and there is no effect at all in kex2 mutants. Such results indicate that protein Scw4p has glucanase activity and its active form is a processed form, wherein a greater activation effect is achieved with Kex2p proteases and lower one with yapsine proteases.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:311458
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-27 13:53:01