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master's thesis
Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:332334
Štanfel, Lana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Štanfel, L. (2024). Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:332334

Štanfel, Lana. "Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:332334

Štanfel, Lana. "Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:332334

Štanfel, L. (2024). 'Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:332334

Štanfel L. Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2025 January 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:332334

L. Štanfel, "Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:332334

Metadata
TitleRazvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata
Title (english)Development of HPLC method for analysis of antibiotic doxycycline hyclate and its degradation products
AuthorLana Štanfel
MentorAntonija Trontel (mentor)
MentorAnita Šporec (pomoć pri izradi, neposredni voditelj)
Committee memberMario Novak (predsjednik povjerenstva)
Committee memberAndreja Leboš-Pavunc (član povjerenstva)
Committee memberBlaženka Kos (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2024-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Doksiciklin hiklat je jedan od često upotrebljavanih antibiotika za liječenje različitih bolesti kod ljudi i životinja. Stoga je neophodno ispuniti zahtjeve o kvaliteti lijeka kao što je mikrobiološka čistoća u cilju postizanja sigurnosti primjene lijeka. Uobičajeni pristupi inaktivacije antibiotika uključuju razrjeđivanje do niskih koncentracija ili kemijsku razgradnju, dok se u ovom radu istražuje enzimska razgradnja kao ekološki prihvatljivija alternativa. Odabran je enzim lakaza koji oksidacijom fenolnih skupina razgrađuje doksiciklin hiklat prisutan u farmaceutskom proizvodu odnosno tableti. Razvijena je gradijentna HPLC metoda za praćenje promjene koncentracije doksiciklin hiklata i srodnih spojeva. Primjenom Luna Omega Polar C18 kolone (150 x 3 mm, 3 µm) s mobilnom fazom koja se sastoji od acetatnog pufera pH=8,8 i acetonitrila, uz protok 0,8 mL/min i pri 274 nm, eluira se doksiciklin hiklat i njegovi razgradni spojevi unutar 25 minuta. Metodom se može učinkovito pratiti enzimska razgradnja, što je ključno za procjenu djelotvornosti lakaze u razgradnji antibiotika.
Abstract (english)
Doxycycline hyclate is one of the most commonly used antibiotics for treating various diseases in humans and animals. Therefore, it is essential to meet the quality requirements of the drug, such as microbiological purity, to ensure the safety of its application. Traditional approaches to antibiotic inactivation include dilution to low concentrations or chemical degradation, while this study investigates enzymatic degradation as a more environmentally friendly alternative. The enzyme laccase, which oxidizes phenolic groups, was selected to degrade doxycycline hyclate present in a pharmaceutical product (tablet). A gradient HPLC method was developed to monitor changes in the concentration of doxycycline hyclate and related compounds. Using a Luna Omega Polar C18 column (150 x 3 mm, 3 µm) with a mobile phase consisting of acetate buffer pH=8.8 and acetonitrile, at a flow rate of 0.8 mL/min and detection at 274 nm, doxycycline hyclate and its degradation products elute within 25 minutes. This method can effectively monitor the enzymatic degradation, which is crucial for assessing the effectiveness of laccase in the degradation of the antibiotic.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:332334
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-10-17 12:13:46