prikaz prve stranice dokumenta Fitotoksičnost i citotoksičnost hidrofobnih niskotemperaturnih eutektičkih otapala
  Download
PDF 996.13 KB
master's thesis
Fitotoksičnost i citotoksičnost hidrofobnih niskotemperaturnih eutektičkih otapala
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:828676
Železnjak, Martina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Železnjak, M. (2024). Fitotoksičnost i citotoksičnost hidrofobnih niskotemperaturnih eutektičkih otapala (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:828676

Železnjak, Martina. "Fitotoksičnost i citotoksičnost hidrofobnih niskotemperaturnih eutektičkih otapala." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:828676

Železnjak, Martina. "Fitotoksičnost i citotoksičnost hidrofobnih niskotemperaturnih eutektičkih otapala." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:828676

Železnjak, M. (2024). 'Fitotoksičnost i citotoksičnost hidrofobnih niskotemperaturnih eutektičkih otapala', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:828676

Železnjak M. Fitotoksičnost i citotoksičnost hidrofobnih niskotemperaturnih eutektičkih otapala [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:828676

M. Železnjak, "Fitotoksičnost i citotoksičnost hidrofobnih niskotemperaturnih eutektičkih otapala", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:828676

Metadata
TitleFitotoksičnost i citotoksičnost hidrofobnih niskotemperaturnih eutektičkih otapala
Title (english)Phytotoxicity and cytotoxicity of hydrophobic deep eutectic solvents
AuthorMartina Železnjak
MentorIvana Radojčić Redovniković (mentor)
MentorMartina Bagović Kolić (pomoć pri izradi, neposredni voditelj)
Committee memberKristina Radošević (predsjednik povjerenstva)
Committee memberIvana Kmetič (član povjerenstva)
Committee memberKsenija Durgo (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2024-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Niskotemperaturna eutektička otapala (engl. Deep Eutectic Solvents, DES) imaju velik potencijal za uporabu u industriji zbog svoje nehlapivosti, nezapaljivosti, stabilnosti, jednostavne pripreme i niske cijene početnih sirovina. Glavnina opisanih DES-ova je hidrofilnog karaktera, no zadnjih desetak godina u porastu je i broj publikacija o hidrofobnim DES-ovima, što pokazuje veliki interes za iste u znanstvenom svijetu. U ovom radu ispitana je toksičnost DES-ova timol:dekadska kiselina (1:3), timol:mentol (1:1) i mentol:oktanska kiselina (1:1) na staničnim linijama HaCaT i HeLa, te na sjemenkama obične pšenice (Triticum aestivum sp.). Pšenica je tretirana DES-ovima u rasponu koncentracija od 5 do 500 mg L⁻¹ pri čemu je praćena germinacija, duljina izdanaka te aktivnost antioksidativnih enzima. Stanične linije tretirane su u koncentracijama od 100 do 200 mg L⁻¹, pri čemu nije došlo do ispoljavanja citotoksičnih učinaka.
Abstract (english)
Deep eutectic solvents (DESs) have a great potential for industrial use due to their non volatility, non-flammability, stability, easy preparation and low cost of starting raw materials. Most of the described deep eutectic solvents are hydrophilic in nature, but the number of publications on hydrophobic DESs has been increasing in the last ten years, which shows a great interest for their development in the scientific world. In this work, the toxicity of DESs thymol:octanoic acid (1:3), thymol:menthol (1:1) and menthol:decanoic acid (1:1) was tested on HaCaT and HeLa cell lines, and on wheat seeds (Triticum aestivum sp.). Wheat was treated with DES in the concentration range from 5 to 500 mg L⁻¹, where germination, length of shoots and activity of antioxidant enzymes were monitored. The cell lines were treated at concentrations of 100 to 200 mg L⁻¹, and no cytotoxic effects were observed.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:828676
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-10-21 12:24:43