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master's thesis
Utjecaj kurkumina i pirazolnog analoga kurkumina u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju stanica raka dojke
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:020997
Režek, Laura
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Toxicology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Režek, L. (2024). Utjecaj kurkumina i pirazolnog analoga kurkumina u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju stanica raka dojke (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:020997

Režek, Laura. "Utjecaj kurkumina i pirazolnog analoga kurkumina u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju stanica raka dojke." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:020997

Režek, Laura. "Utjecaj kurkumina i pirazolnog analoga kurkumina u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju stanica raka dojke." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:020997

Režek, L. (2024). 'Utjecaj kurkumina i pirazolnog analoga kurkumina u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju stanica raka dojke', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:020997

Režek L. Utjecaj kurkumina i pirazolnog analoga kurkumina u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju stanica raka dojke [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:020997

L. Režek, "Utjecaj kurkumina i pirazolnog analoga kurkumina u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju stanica raka dojke", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:020997

Metadata
TitleUtjecaj kurkumina i pirazolnog analoga kurkumina u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju stanica raka dojke
Title (english)The effect of curcumin and pyrazole analogue of curcumin under conditions of nutrient deprivation on the viability and proliferation of breast cancer cells
AuthorLaura Režek
MentorIvana Kmetič (mentor)
MentorMarina Miletić (pomoć pri izradi, neposredni voditelj)
MentorTeuta Murati (pomoć pri izradi, neposredni voditelj)
Committee memberTeuta Murati (predsjednik povjerenstva)
Committee memberKristina Radošević (član povjerenstva)
Committee memberIgor Slivac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Toxicology)
Zagreb
Defense date and country2024-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj rada bio je ispitati učinak nutritivne deprivacije, kao i učinak kurkumina (CRC) i pirazolnog analoga kurkumina (PC) (2,5 – 100 µM) u uvjetima nutritivne deprivacije na vijabilnost i proliferaciju MCF 7 stanične linije, te na indukciju stanične smrti autofagijom i apoptozom. Stanična vijabilnost praćena je metodama MTT, Neutral Red i Trypan Blue, dok je analiza stanične smrti provedena metodom protočne citometrije. Uzgoj stanica u DMEM High Glucose i DMEM Low Glucose hranjivim medijima razrijeđenim PBS ili HBSS puferom uzrokovao je značajno smanjenje proliferacije stanica određeno MTT metodom, pri čemu koncentracija dostupne glukoze nije bila ograničavajući parametar. Nutritivna deprivacija potaknula je autofagiju kod samo jednog uzorka, dok je apoptoza bila blago inducirana kod svih i to nešto jače kod primjene medija s nižom koncentracijom glukoze. CRC i PC uzrokovali su smanjenje stanične vijabilnosti u ovisnosti u dozi, pri čemu su se parametri poput načina i vremena tretiranja stanica (stanice nacijepljene u mediju za uzgoj u koji je dodan CRC ili PC; stanice tretirane s CRC-om ili PC-om nakon 24h (odnosno nakon formiranog monosloja)) pokazali važnim za konačne citotoksične učinke navedenih spojeva. Nutritivna deprivacija pojačala je citotoksično djelovanje CRC-a, što je potvrđeno metodama MTT, Neutral Red i Trypan Blue. Udio stanica koje odumiru autofagijom ili apoptozom značajno je porastao povećanjem koncentracije CRC-a (5 – 100 µM) u uvjetima nutritivne deprivacije.
Abstract (english)
The aim of this study was to examine the effect of nutrient deprivation, as well the effect of curcumin (CRC) and pyrazole analogue of curcumin (PC) (2,5 – 100 µM) under conditions of nutrient deprivation on the viability and proliferation of the MCF-7 cells, and on the induction of cell death by autophagy and apoptosis. Cell viability was determined using MTT, Neutral Red and Trypan Blue methods, while cell death analysis was performed using the flow cytometry method. DMEM High Glucose and DMEM Low Glucose media diluted with PBS or HBSS caused a significant decrease in cell proliferation, which was determined by MTT method, while the concentration of available glucose was not the limiting parameter. Nutrient deprivation stimulated autophagy in only one sample, while apoptosis was mildly induced in all of them and somewhat stronger when using media with a lower glucose concentration. CRC and PC caused a decrease in cell viability in a dose-dependent manner, with parameters such as conditions and time of cell treatment (cells inoculated in culture medium to which CRC or PC was added; cells treated with CRC or PC after 24h (that is, after monolayer formation)) proved to be important for the final cytotoxic effects of the mentioned compounds. Nutrition deprivation enhanced the cytotoxic effect of CRC, which was confirmed by the MTT, Neutral Red and Trypan Blue methods. The proportion of cells that undergo cell death by autophagy or apoptosis increased significantly in conditions of nutrient deprivation as concentration of CRC (5 – 100 µM) increased.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:020997
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-10-21 12:48:06