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master's thesis
Biološka aktivnost konjugata ferocena i 2-tiouracila određena na HeLa staničnoj liniji
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:922271
Fišić, Katarina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Fišić, K. (2024). Biološka aktivnost konjugata ferocena i 2-tiouracila određena na HeLa staničnoj liniji (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:922271

Fišić, Katarina. "Biološka aktivnost konjugata ferocena i 2-tiouracila određena na HeLa staničnoj liniji." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:922271

Fišić, Katarina. "Biološka aktivnost konjugata ferocena i 2-tiouracila određena na HeLa staničnoj liniji." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:922271

Fišić, K. (2024). 'Biološka aktivnost konjugata ferocena i 2-tiouracila određena na HeLa staničnoj liniji', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 17 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:922271

Fišić K. Biološka aktivnost konjugata ferocena i 2-tiouracila određena na HeLa staničnoj liniji [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2024 November 17] Available at: https://urn.nsk.hr/urn:nbn:hr:159:922271

K. Fišić, "Biološka aktivnost konjugata ferocena i 2-tiouracila određena na HeLa staničnoj liniji", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:922271

Metadata
TitleBiološka aktivnost konjugata ferocena i 2-tiouracila određena na HeLa staničnoj liniji
Title (english)Biological activity of ferrocene and 2-thiouracil conjugates assessed on the HeLa cell line
AuthorKatarina Fišić
MentorKristina Radošević (mentor)
MentorMartina Bagović Kolić (pomoć pri izradi, neposredni voditelj)
Committee memberJasmina Lapić (predsjednik povjerenstva)
Committee memberTeuta Murati (član povjerenstva)
Committee memberSenka Djaković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2024-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ferocenski konjugati su organometalni spojevi sa specifičnim elektrokemijskim i biološkim svojstvima, što ih čini zanimljivima za istraživanje u razvoju novih lijekova. Budući da mnogi od njih pokazuju antitumorski učinak, u ovom radu je ispitana in vitro aktivnost tri biokonjugata ferocena i 2 tiouracila na HeLa stanicama praćenjem stanične vijabilnosti te klonogenom analizom. Nadalje, protočnom citometrijom određeno je s kojim se staničnim procesom može povezati zapažena citotoksičnost. Ispitani biokonjugati ferocena i 2-tiouracila djeluju inhibitorno na rast HeLa stanica pri čemu je inhibitorni učinak ovisan o dozi. Najsnažniji citotoksični učinak pokazuje spoj 1 sa IC₅₀ vrijednošću 23,39 μM. Inhibitorni učinak može se povezati s poticanjem stanične smrti mehanizmom apoptoze. Slijedom navedenog može se zaključiti da biokonjugati ferocena i 2-tiouracila imaju potencijal kao antitumorski agensi.
Abstract (english)
Ferrocene conjugates are organometallic compounds with specific electrochemical and biological properties, what makes them interesting in drug development research. Since many of them exhibit antitumor effects, in this study the in vitro activity of three ferrocene and 2-thiouracil bioconjugates on HeLa cells was examined by cell viability and clonogenic assays. Furthermore, flow cytometry was used to determine which cellular processes are associated with the observed cytotoxicity. The tested bioconjugates of ferrocene and 2-thiouracil have an inhibitory effect on the growth of HeLa cells, which is dose-dependent. The strongest cytotoxic effect is shown by compound 1 with an IC₅₀ value of 23.39 μM. The inhibitory effect can be related to the induction of cell death by the apoptosis. Therefore, it can be concluded that bioconjugates of ferrocene and 2-thiouracil have potential as antitumor agents.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:922271
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-10-24 09:43:59