prikaz prve stranice dokumenta Inflammasome dependent skin inflammation in psoriasis
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master's thesis
Inflammasome dependent skin inflammation in psoriasis
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:078043
Vuksan, Lara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vuksan, L. (2024). Inflammasome dependent skin inflammation in psoriasis (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:078043

Vuksan, Lara. "Inflammasome dependent skin inflammation in psoriasis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:078043

Vuksan, Lara. "Inflammasome dependent skin inflammation in psoriasis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:078043

Vuksan, L. (2024). 'Inflammasome dependent skin inflammation in psoriasis', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:078043

Vuksan L. Inflammasome dependent skin inflammation in psoriasis [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:078043

L. Vuksan, "Inflammasome dependent skin inflammation in psoriasis", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:078043

Metadata
TitleInflammasome dependent skin inflammation in psoriasis
Title (croatian)Upala kože kod psorijaze ovisna o inflamasomu
AuthorLara Vuksan
MentorIgor Stuparević (mentor)
Committee memberVišnja Besendorfer (član povjerenstva)
Committee memberMaja Matulić (član povjerenstva)
Committee memberIgor Stuparević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2024-07-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Psoriasis is a chronic, systemic, immune-mediated disease characterised by keratinocyte hyperproliferation, infiltration of immune cells into the dermis and epidermis and an excessive inflammatory signaling that is creating a positive loop. In this study we aimed to assess the role of NLRP6 and GSDMD in the pathophysiology of psoriasis, using an imiquimod murine model of psoriasis. Bioinformatic analysis confirmed the already reported involvement of inflammasome and pyroptosome complexes in human psoriasis and mouse imiquimod induced psoriasis-like inflammation. We confirmed that there is an induced pro- and anti inflammatory response in in vitro bone marrow-derived macrophages activated with imiquimod (IMQ). In vivo study suggested there might be some differential trends between wild type and knockout mice treated with IMQ, however these trends would have to be evaluated by repeating the study with a greater number of mice. Future research should involve in vitro HaCaT culture and flow cytometry analysis.
Abstract (croatian)
Psorijaza je kronična, sistemska i imunološki posredovana bolest karakterizirana hiperproliferacijom keratinocita, infiltracijom imunoloških stanica u dermis i epidermis te pretjeranim upalnim signaliziranjem koje stvara pozitivnu spregu. U ovom smo istraživanju željeli odrediti ulogu gena NLRP6 i GSDMD u patofiziologiji psorijaze, koristeći imikvimod model psorijaze na miševima. Bioinformatička analiza potvrdila je već zabilježenu uključenost inflamasoma i piropotosoma u ljudskoj psorijazi i mišjoj upali induciranoj imikvimodom. Potvrdili smo da postoji pro- i anti-upalni odgovor u in vitro kulturi makrofaga izoliranih iz koštane srži i aktiviranih imikvimodom (IMQ). In vivo istraživanje sugeriralo je da postoje mogući različiti trendovi između divljih i knockout miševa tretiranih sa imikvimodom. Međutim, spomenuto je potrebno dodatno procijeniti ponavljanjem istraživanja s većim brojem miševa. Buduća istraživanja trebala bi uključiti in vitro HaCaT kulturu i analizu protočnom citometrijom.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:159:078043
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2024-11-07 10:44:14