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master's thesis
Optimizacija uvjeta uzgoja kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp. na otpadnoj morskoj travi
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:204218
Morača, Nika
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Morača, N. (2024). Optimizacija uvjeta uzgoja kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp. na otpadnoj morskoj travi (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:204218

Morača, Nika. "Optimizacija uvjeta uzgoja kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp. na otpadnoj morskoj travi." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:204218

Morača, Nika. "Optimizacija uvjeta uzgoja kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp. na otpadnoj morskoj travi." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:204218

Morača, N. (2024). 'Optimizacija uvjeta uzgoja kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp. na otpadnoj morskoj travi', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:204218

Morača N. Optimizacija uvjeta uzgoja kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp. na otpadnoj morskoj travi [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:204218

N. Morača, "Optimizacija uvjeta uzgoja kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp. na otpadnoj morskoj travi", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:204218

Metadata
TitleOptimizacija uvjeta uzgoja kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp. na otpadnoj morskoj travi
Title (english)Optimization of cultivating conditions of the yeast Rhodotorula sp. and the thraustochytrid Schizochytrium sp. on seagrass waste
AuthorNika Morača
MentorMirela Ivančić Šantek (mentor)
Committee memberBlaženka Kos (predsjednik povjerenstva)
Committee memberTibela Landeka Dragičević (član povjerenstva)
Committee memberJasna Novak (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2024-11-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Sažetak: S obzirom na iscrpljivanje prirodnih resursa poput fosilnih goriva, postoji velika potreba za pronalaskom obnovljivih izvora energije. Lignocelulozna biomasa, kompleksni polimer, obećavajuća je obnovljiva sirovina koja se može koristiti za proizvodnju bioenergetskih i drugih produkata pomoću različitih tipova mikroorganizama. Cilj ovog rada je optimizacija omjera ugljika i dušika (C:N) u hranjivoj podlozi za uzgoj dva oleaginozna mikroorganizma – kvasca Rhodotorula sp. i traustohitrida Schizochytrium sp.. U nastavku istraživanja proveden je uzgoj navedenih mikroorganizama na predobrađenoj otpadnoj travi koja je pretežito sadržavala morsku travu Posidonia oceanica. Najbolji C:N omjer za rast biomase i prinos lipida bio je 100 g/g za kvasac te 3,75 g/g za traustohitrid. Uzgojem kvasca na predobrađenoj morskoj travi proizvedeno je 73,4 g lipida/kg sirovine, a uzgojem traustohitrida 132,2 g lipida/kg sirovine.
Abstract (english)
Abstract: As natural resources such as fossil fuels deplete, there is an urgent need to find sustainable energy sources. Lignocellulose, a complex polymer, has emerged as a promising renewable resource which can be used for production of bioenergetic and other products using different kinds of microorganisms. The goal of this thesis is optimization of carbon to nitrogen (C:N) ratio for cultivation of two oleaginous microorganisms – yeast Rhodotorula sp. and thraustochytrid Schizochytrium sp. using the marine seagrass Posidonia oceanica as a substrate. In the following research, the cultivation of mentioned microorganisms was carried out on pretreated waste grass, which predominantly contained Posidonia oceanica. The best C:N ratio for biomass growth and lipid yield was 100 g/g for the yeast and 3,75 g/g for the thraustochytrid. Cultivation of yeast on pretreated sea grass yielded 73,4 g of lipids/kg of raw material and cultivation of thraustochytrid yielded 132,2 g of lipids/kg of raw material.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:204218
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-12-04 09:58:58