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master's thesis
Određivanje ostataka neomicina u hrani životinjskog podrijetla
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:573159
Barišić, Danica
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Barišić, D. (2014). Određivanje ostataka neomicina u hrani životinjskog podrijetla (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:573159

Barišić, Danica. "Određivanje ostataka neomicina u hrani životinjskog podrijetla." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:573159

Barišić, Danica. "Određivanje ostataka neomicina u hrani životinjskog podrijetla." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:573159

Barišić, D. (2014). 'Određivanje ostataka neomicina u hrani životinjskog podrijetla', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 13 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:573159

Barišić D. Određivanje ostataka neomicina u hrani životinjskog podrijetla [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2025 January 13] Available at: https://urn.nsk.hr/urn:nbn:hr:159:573159

D. Barišić, "Određivanje ostataka neomicina u hrani životinjskog podrijetla", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:573159

Metadata
TitleOdređivanje ostataka neomicina u hrani životinjskog podrijetla
Title (english)Determination of neomycin in animal food
AuthorDanica Barišić
MentorJagoda Šušković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2014-05-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Zbog raširene primjene antibakterijskih lijekova, mora se kontrolirati prisutnost rezidua u hrani životinjskog podrijetla. Kontrola antibiotika u hrani potrebna je kao jedna od temeljnih odrednica u službi očuvanja zdravlja ljudi. Cilj ovog rada bio je odrediti koncentracije ostataka (rezidua) neomicina u mlijeku i mišićnom tkivu domaćih životinja. Koncentracija neomicina u uzorcima je određivana ELISA metodom. Prema dobivenim rezultatima, niti u jednom ispitanom uzorku određena koncentracija neomicina nije prelazila vrijednost najveće dopuštene količine (NDK) za neomicin propisane Uredbom Europske komisije 37/2010 o najvećoj dopuštenoj količini (NDK) ostataka antibiotika u hrani životinjskog podrijetla. Također je pokazano da je ELISA pozudana metoda za određivanje rezidua neomicina u uzorcima hrane životinjskog podrijetla. Da su uzorci bili pozitivni na neomicin, u tom bi slučaju bilo potrebno primjeniti jednu od metoda za potvrdu kojima se identitet rezidua i njegova koncentracija potvrđuju s najvećom mogućom preciznošću i točnošću, kao što su LC–MS/MS i GC–MS/MS.
Abstract (english)
Because of the widespread use of antibacterial drugs, must be controlled the presence of residues in food of animal origin. Control of antibiotics in food is needed as one of the basic elements in the service of preserving the health of people. The aim of this study was to determine the concentrations of residues (residue) of neomycin in milk and muscle tissue of animals. Neomycin concentration in samples was determined by ELISA method. According to the results, in any sample examined the concentration of neomycin did not exceed the value of the maximum levels (MRLs) for neomycin prescribed in the Council Regulation 37/2010/EU on pharmacologically active substances and their classification regarding maximum residue limits in food stuffs of animal origin. It was also shown that the ELISA greedy method for determining residues of neomycin in food samples of animal origin. That the samples were positive to neomycin, in this case it would be necessary to apply one method to confirm that the identity of the residue and its concentration is confirmed with the utmost precision and accuracy, such as LC-MS/MS and GC-MS/MS.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:573159
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 10:01:58