prikaz prve stranice dokumenta Intracellular trafficking of pDNA. Cytosolic migration and nuclear import
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master's thesis
Intracellular trafficking of pDNA. Cytosolic migration and nuclear import
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:370880
Živković, Martina
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Živković, M. (2014). Intracellular trafficking of pDNA. Cytosolic migration and nuclear import (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:370880

Živković, Martina. "Intracellular trafficking of pDNA. Cytosolic migration and nuclear import." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:370880

Živković, Martina. "Intracellular trafficking of pDNA. Cytosolic migration and nuclear import." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:370880

Živković, M. (2014). 'Intracellular trafficking of pDNA. Cytosolic migration and nuclear import', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 12 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:370880

Živković M. Intracellular trafficking of pDNA. Cytosolic migration and nuclear import [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 July 12] Available at: https://urn.nsk.hr/urn:nbn:hr:159:370880

M. Živković, "Intracellular trafficking of pDNA. Cytosolic migration and nuclear import", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:370880

Metadata
TitleIntracellular trafficking of pDNA. Cytosolic migration and nuclear import
AuthorMartina Živković
MentorVladimir Mrša (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2014-07-04, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Gene therapy provides a great hope for the treatment of many acquired and inherited life-threatening diseases. Nucleic acid drugs, however, must be delivered to the interior of the target cell while surviving an array of biological barriers. Successful gene therapy is thus dependent on the development of an efficient delivery vector. Nonviral vectors, as an attractive alternative to viral vectors, have been intensively investigated for decades; advantages such as a potentially improved safety profile, flexibility in the size of the delivered gene and lower immunogenicity have all been demonstrated. One of the most critical biological barriers for successfully delivery of pDNA with non-viral vectors is a cytoplasmic transport and nuclear import. In this work, to overcome these barriers, I performed the transfection of different cell lines using polyplexes and lipoplexes combined with two signals which allow easier cytoplasmic trafficking and nuclear import of pDNA. For the successful delivery of pDNA towards the nucleus, we investigated the possibility of peptide P79-98 for docking pDNA onto microtubules. For nuclear import we explored the capacity of 3NF sequence inserted in pDNA which is recognized by the transcription factor NFκB and shuttled into nucleus.
Keywords
Languageenglish
URN:NBNurn:nbn:hr:159:370880
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 11:42:15