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master's thesis
Istraživanje citotoksičnog, prooksidativnog i mutagenog učinka auksina na ljudske stanice raka debelog crijeva
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:795046
Bagarić, Ena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bagarić, E. (2014). Istraživanje citotoksičnog, prooksidativnog i mutagenog učinka auksina na ljudske stanice raka debelog crijeva (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:795046

Bagarić, Ena. "Istraživanje citotoksičnog, prooksidativnog i mutagenog učinka auksina na ljudske stanice raka debelog crijeva." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:795046

Bagarić, Ena. "Istraživanje citotoksičnog, prooksidativnog i mutagenog učinka auksina na ljudske stanice raka debelog crijeva." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:795046

Bagarić, E. (2014). 'Istraživanje citotoksičnog, prooksidativnog i mutagenog učinka auksina na ljudske stanice raka debelog crijeva', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:795046

Bagarić E. Istraživanje citotoksičnog, prooksidativnog i mutagenog učinka auksina na ljudske stanice raka debelog crijeva [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:795046

E. Bagarić, "Istraživanje citotoksičnog, prooksidativnog i mutagenog učinka auksina na ljudske stanice raka debelog crijeva", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:795046

Metadata
TitleIstraživanje citotoksičnog, prooksidativnog i mutagenog učinka auksina na ljudske stanice raka debelog crijeva
Title (english)Research of cytotoxic, prooxidative and mutagenic effects of auxins on human colorectal carcinoma cells
AuthorEna Bagarić
MentorKsenija Durgo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2014-02-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Auksini su biljni hormoni koji su po kemijskoj strukturi indolni spojevi, a kao takvi mogu posjedovati antikancerogena svojstva. Primjena ove vrste spojeva umnogome bi olakšala terapiju raka jer je riječ o prirodnim i neinvazivnim spojevima prisutnim u biljkama, mikroorganizmima i čovjeku. U ovom radu istraženo je citotoksično, prooksidativno i mutageno djelovanje auksina (indol-3-octena kiselina, 2-metil indol-3-octena kiselina, indol-3-maslačna kiselina i indol-3-propionska kiselina) na stanice raka debelog crijeva (Caco-2). Niti jedan istraživani auksin nije pokazao citotoksični učinak. Otkriveno je da indol-3-octena kiselina (0,01 mM) najintenzivnije djeluje na povećanje stvaranja slobodnih radikala (za 274,89 %), dok peroksidaciju lipida u najvećoj mjeri uzrokuje indol-3-maslačna kiselina (0,5 mM). Niti jedan od auksina nije pokazao mutageno djelovanje. Tretman s indol-3-propionskom kiselinom pokazao je najbliži rezultat onom koji nastaje nakon tretmana s vodikovim peroksidom.
Abstract (english)
Auxins are plant hormones which are, by their structure, indolic compounds and with that comes a possibility that they can have anticancerogenic properties. Application and implementation of these compounds could ease the cancer therapy in huge extent because they are noninvasive, naturaly occuring in plants, microorganisms and humans. In this thesis cytotoxic, prooxidative and mutagenic effect of auxins (indole-3-acetic acid, 2-methyl indole-3-acetic acid, indole-3-butyric acid and indole-3-propionic acid) on human colorectal adenocarcinoma cells (Caco-2) were investigated. None of the tested auxins did show cytotoxic effect. It was discovered that IAA (0,01 mM) caused the highest formation of free radicals (for 274,89 %). On the other hand, the highest peroxidation of lipids was caused by IBA (0,5 mM). None of the auxins did show mutagenic effects. Treatment with indole-3-propionic acid showed the result closest to the one observed after the treatment with hydrogen peroxide.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:795046
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-28 12:02:30