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master's thesis
Utjecaj vodikovog peroksida na heterotrofni uzgoj alge Euglena gracilis
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:055141
Jurković, Ena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Jurković, E. (2014). Utjecaj vodikovog peroksida na heterotrofni uzgoj alge Euglena gracilis (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:055141

Jurković, Ena. "Utjecaj vodikovog peroksida na heterotrofni uzgoj alge Euglena gracilis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:055141

Jurković, Ena. "Utjecaj vodikovog peroksida na heterotrofni uzgoj alge Euglena gracilis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:055141

Jurković, E. (2014). 'Utjecaj vodikovog peroksida na heterotrofni uzgoj alge Euglena gracilis', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:055141

Jurković E. Utjecaj vodikovog peroksida na heterotrofni uzgoj alge Euglena gracilis [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:055141

E. Jurković, "Utjecaj vodikovog peroksida na heterotrofni uzgoj alge Euglena gracilis", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:055141

Metadata
TitleUtjecaj vodikovog peroksida na heterotrofni uzgoj alge Euglena gracilis
Title (english)The effect of hydrogen peroxide on the heterotrophic cultivation of alfae Euglena gracilis
AuthorEna Jurković
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2014-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom istraživanju proučavan je heterotrofni uzgoj alge Euglena gracilis s ciljem definiranja optimalnog sastava hranjive podloge za industrijsku proizvodnju biomase i paramylona. Tijekom uzgoja na laboratorijskoj tresilici i u bioreaktoru s mješalom ispitivan je heterotrofni uzgoj alge E. gracilis na kemijski definiranoj (modificirana Hutnerova podloga) i kompleksnoj podlozi sa i bez dodatka vodikovog peroksida, te sa i bez kontaminacije kvascem Saccharomyces cerevisiae i bakterijom Bacillus subtilis. Kompleksna hranjiva podloga (20 g/L glukoze + 60 g/L CSL) primijenjena je s ciljem smanjenja troškova proizvodnje biomase i paramylona u velikom mjerilu. Na osnovi rezultata istraživanja vidljivo je da kompleksna hranjiva podloga može uspješno zamijeniti kemijski definiranu Hutner-ovu podlogu. Stoga kompleksna hranjiva podloga ima veliki potencijal za primjenu u industrijskoj proizvodnji. Nadalje, kod heterotrofnog uzgoja na obje hranjive podloge uočeno je da 5 mg/L H2O2 ima značajan utjecaj na dinamiku odvijanja bioprocesa. Navedena koncentracija H2O2 uspješno sprječava rast kontaminanata (S. cerevisiae i B. subtilis), a što je dokazano uzgojem u Erlenmeyer tikvicama i u bioreaktoru s mješalom.
Abstract (english)
In this research, the heterotrophic cultivation of algae Euglena gracilis was studied in order to define the optimum composition of culture media for the industrial production of biomass and paramylon. During the heterotrophic cultivation on the laboratory shaker and stirred tank bioreactor, the chemically defined (modified Hutner medium) and a complex medium with and without the addition of hydrogen peroxide, and with and without contamination of the yeast Saccharomyces cerevisiae and the bacterium Bacillus subtilis, were examined. The complex nutrient medium (20 g/L glucose + 60 g/L CSL) was applied in order to reduce production costs on a large scale. Based on the results of research, it is evident that a complex nutrient medium can successfully replace chemically defined Hutner medium. Therefore, a complex nutrient medium has a great potential for application in industrial production. Furthermore, in heterotrophic cultivation on both culture media was observed that 5 mg/L H2O2 has a significant impact on the dynamics of bioprocess. The above mentioned concentration of H2O2 successfully prevents the growth of contaminants (S. cerevisiae and B. subtilis), which is proven by cultivation in Erlenmeyer flasks and stirred tank bioreactor.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:055141
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2017-04-28 12:25:32