prikaz prve stranice dokumenta Utjecaj kisika i ugljikovog dioksida na rast i aktivnost bakterije mliječne kiseline Lactobacillus coryniformis subsp. torquens DSM 20004T
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master's thesis
Utjecaj kisika i ugljikovog dioksida na rast i aktivnost bakterije mliječne kiseline Lactobacillus coryniformis subsp. torquens DSM 20004T
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:318898
Hviezd, Katarina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Hviezd, K. (2014). Utjecaj kisika i ugljikovog dioksida na rast i aktivnost bakterije mliječne kiseline Lactobacillus coryniformis subsp. torquens DSM 20004T (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:318898

Hviezd, Katarina. "Utjecaj kisika i ugljikovog dioksida na rast i aktivnost bakterije mliječne kiseline Lactobacillus coryniformis subsp. torquens DSM 20004T." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:318898

Hviezd, Katarina. "Utjecaj kisika i ugljikovog dioksida na rast i aktivnost bakterije mliječne kiseline Lactobacillus coryniformis subsp. torquens DSM 20004T." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:318898

Hviezd, K. (2014). 'Utjecaj kisika i ugljikovog dioksida na rast i aktivnost bakterije mliječne kiseline Lactobacillus coryniformis subsp. torquens DSM 20004T', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:318898

Hviezd K. Utjecaj kisika i ugljikovog dioksida na rast i aktivnost bakterije mliječne kiseline Lactobacillus coryniformis subsp. torquens DSM 20004T [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:159:318898

K. Hviezd, "Utjecaj kisika i ugljikovog dioksida na rast i aktivnost bakterije mliječne kiseline Lactobacillus coryniformis subsp. torquens DSM 20004T", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:318898

Metadata
TitleUtjecaj kisika i ugljikovog dioksida na rast i aktivnost bakterije mliječne kiseline Lactobacillus coryniformis subsp. torquens DSM 20004T
Title (english)Effect of oxygen and carbon dioxide on the growth and activity of lactic acid bacterium Lactobacillus coryniformis subsp. torquens 20004T
AuthorKatarina Hviezd
MentorAnita Slavica (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2014-09-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Visoka koncentracija kisika, koji je otopljen u suspenziji, pospješuje iscrpljivanje glukoze iz hranjive MRS podloge s pomoću soja Lactobacillus coryniformis subsp. torquens DSM 20004T. Smanjena koncentracija kisika i dobava ugljikovog dioksida pogoduju sintezi biomase ovog bakterijskog soja i proizvodnji D-(-)-laktata (P1) (YP1/S = 0,94 g/g), jednog od dva krajnja proizvoda metabolizma glukoze. Fakultativno heterofermentativni soj L. coryniformis subsp. torquens DSM 20004T tijekom rasta u hranjivoj MRS podlozi, koja sadrži relativno visoku koncentraciju acetata (c0 = 60,5 mmol/L), proizvodi i acetat kao krajnji proizvod metabolizma (P2). Na proizvodnju acetata utječu koncentracija glukoze kao i koncentracija kisika i ugljikovog dioksida u podlozi. Kao načini regulacije metabolizma glukoze i proizvodnje D-(-)-laktata i acetata kod ove Gram-pozitivne bakterije identificirani su i okarakterizirani (1) „acetatni prekidač“ i (2) limitacija glukozom (cS < 42,0 – 50,0 mmol/L).
Abstract (english)
Depletion of glucose from MRS medium by Lactobacillus coryniformis subsp. torquens DSM 20004T was enhanced at relatively high concentration of dissolved oxygen. Synthesis of bacterial biomass and production of D-(-)-lactate (P1) (YP1/S = 0,94 g/g), one of two end-products of glucose metabolism, were favored at decreased concentration of dissolved oxygen and increased concentration of carbon dioxide. During growth in the MRS medium, which contains relatively high concentration of acetate (c0 = 60,5 mmol/L), species L. coryniformis subsp. torquens DSM 20004T produced second end-product - acetate (P2). Production of acetate was affected by concentration of glucose and concentration of dissolved oxygen and carbon dioxyde. „Acetate switch“ and glucose limitation (cS < 42,0 – 50,0 mmol/L) were identified and characterized as mechanisms of regulation of glucose metabolism and production of D-(-)-lactate and acetate by this Gram-positive bacterium.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:318898
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 08:25:00