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undergraduate thesis
Gubitak heterozigotnosti tumor supresurskog gena TP53 u meningeomima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:176920
Gunjević, Veronika
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Gunjević, V. (2015). Gubitak heterozigotnosti tumor supresurskog gena TP53 u meningeomima (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:176920

Gunjević, Veronika. "Gubitak heterozigotnosti tumor supresurskog gena TP53 u meningeomima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:176920

Gunjević, Veronika. "Gubitak heterozigotnosti tumor supresurskog gena TP53 u meningeomima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:176920

Gunjević, V. (2015). 'Gubitak heterozigotnosti tumor supresurskog gena TP53 u meningeomima', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 17 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:176920

Gunjević V. Gubitak heterozigotnosti tumor supresurskog gena TP53 u meningeomima [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 July 17] Available at: https://urn.nsk.hr/urn:nbn:hr:159:176920

V. Gunjević, "Gubitak heterozigotnosti tumor supresurskog gena TP53 u meningeomima", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:176920

Metadata
TitleGubitak heterozigotnosti tumor supresurskog gena TP53 u meningeomima
Title (english)Loss of heterozygosity of tumor suppressor gene TP53 in meningioma
AuthorVeronika Gunjević
MentorReno Hrašćan (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2015-09-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Interdisciplinary Technical Sciences
Abstract
U ovom je radu proučavana učestalost pojave gubitka heterozigotnosti gena TP53 kod osoba koje boluju od meningeoma. U tu svrhu, korištena metoda bila je analiza polimorfizma u duljini restrikcijskih fragmenata. Kao uzorak korištena je genomska DNA iz meningeoma i iz leukocita. Najprije je umnožen dio introna 6 gena TP53 PCR-om iz uzoraka, a zatim je vršena restrikcija PCR produkta endonukleazom MspI. Rezultati su bili prikazani elektroforezom na agaroznom gelu. Samo je 21,05% pacijanata u ovom uzorku bilo informativno za ovaj genski marker. U niti jednog pacijenta nije pronađen gubitak heterozigotnosti. Međutim, pošto je uzorak heterozigotnih pacijenata malen, bilo bi dobro istražiti ovu pojavu na većem broju heterozigotnih pacijanata.
Abstract (english)
This paper studied the incidence of loss of heterozygosity of TP53 gene in patients suffering from meningioma. For this purpose, the method used was the Restriction Fragment Length Polymorphism analysis on samples of genomic DNA from meningioma and leukocytes. First, part of intron 6 of gene TP53 was amplified by PCR, and than the restriction of PCR products was performed with MspI endonuclease. Results were obtained by electrophoresis on agarose gel. Only 21,05% of patients were informative for this genetic marker. No patient showed loss of heterozygosity. However, since the sample of heterozygous patients is small, it is necessary to investigate this occurrence on a larger number of heterozygous patients.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:176920
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2016-02-01 11:25:17