prikaz prve stranice dokumenta Proizvodnja lipida s kvascem Trichosporon oleaginosus na kukuruznim oklascima
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master's thesis
Proizvodnja lipida s kvascem Trichosporon oleaginosus na kukuruznim oklascima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:396510
Bakija, Ivana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bakija, I. (2015). Proizvodnja lipida s kvascem Trichosporon oleaginosus na kukuruznim oklascima (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:396510

Bakija, Ivana. "Proizvodnja lipida s kvascem Trichosporon oleaginosus na kukuruznim oklascima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:396510

Bakija, Ivana. "Proizvodnja lipida s kvascem Trichosporon oleaginosus na kukuruznim oklascima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:396510

Bakija, I. (2015). 'Proizvodnja lipida s kvascem Trichosporon oleaginosus na kukuruznim oklascima', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 07 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:396510

Bakija I. Proizvodnja lipida s kvascem Trichosporon oleaginosus na kukuruznim oklascima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 August 07] Available at: https://urn.nsk.hr/urn:nbn:hr:159:396510

I. Bakija, "Proizvodnja lipida s kvascem Trichosporon oleaginosus na kukuruznim oklascima", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:396510

Metadata
TitleProizvodnja lipida s kvascem Trichosporon oleaginosus na kukuruznim oklascima
Title (english)Lipid production on corn cob by yeast Trichosporon oleaginosus
AuthorIvana Bakija
MentorMirela Ivančić - Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2015-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Kvasac Trichosporon oleaginousus primijenjen je u procesu istovremenog ošećerenja i proizvodnje lipida (SSF) s predobrađenim kukuruznim oklascima uz omjer tekućeg i čvrstog dijela podloge (LSR) 20:1-8:1 mL g-1. Nakon 144 sata šaržnog uzgoja, produktivnost nakupljanja lipida povećavala se s povećanjem omjera LSR do maksimalne vrijednosti od 1,69 g L-1 d-1 uz omjer LSR od 8:1 mLg-1. Najveća koncentracija lipida od 11,5 g L-1 postignuta je uz omjer LSR od 8:1 mL g-1 nakon 312 sati uzgoja, dok je najveći udjel lipida 172,8 mg g-1 taloga koji je sadržavao nehidrolizirani supstrat i biomasu kvasca postignut uz LSR of 10:1 mL g-1 nakon 240 h. Nadalje, proveden je šaržni SSF uz prihranu supstratom u obrocima po 2,5 i 5 g. Šaržnom prihranom postignut je ukupni omjer LSR od 5:1 i 6,7:1 mL g-1. Primijenjena strategija prihrane supstratom omogućila je učinkovitije i ravnomjernije miješanje podloge za uzgoj. Kao posljedica postignute su velike koncentracije lipida u podlozi od 26,48 g L-1, udjel lipida od 269,25 mg g-1 i produktivnost od 2,04 g L-1 d-1.
Abstract (english)
Yeast Trichosporon oleaginousus was used for the simultaneous saccharification and lipid production process with pretreated corn cob with liquid to solid ratio (LSR) from 20:1 to 8:1 mLg-1. After 144 hours of batch cultivation, lipid productivity increased with the increase of LSR to the highest value of 1,69 gL-1d-1 at the LSR of 8:1 mLg-1. The highest lipid concentration of 11,5 gL-1 was obtained at LSR of 8:1 mL g-1 after 312 h, while the highest lipid content in total mass containing cells and unhydroyzed substrate of 172,8 mgg-1 was obtained at the LSR of 10:1 mL g-1 after 240 h. Furthermore SSF fed-batch additions of substrate 2,5 and 5 g. Batch substrate additions resulted in cumulative LSR equivalent to 5:1 and 6,7:1 mLg-1. The applied strategy of substrate addition enabled more vigorous and uniform stirring of cultivation media. This in turn resulted in high lipid concentration, lipid content and productivity of 26,48 gL-1, 269,25 mgg-1 and 2,04 gL-1 d-1, respectively.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:396510
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 09:46:58