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master's thesis
Molekularna farmakologija serotoninskog 5-HT6 receptora, terapeutičke mete u kognitivnim poremećajima: funkcionalne studije in vitro i in vivo
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:182232
Baković, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Baković, P. (2015). Molekularna farmakologija serotoninskog 5-HT6 receptora, terapeutičke mete u kognitivnim poremećajima: funkcionalne studije in vitro i in vivo (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:182232

Baković, Petra. "Molekularna farmakologija serotoninskog 5-HT6 receptora, terapeutičke mete u kognitivnim poremećajima: funkcionalne studije in vitro i in vivo." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:182232

Baković, Petra. "Molekularna farmakologija serotoninskog 5-HT6 receptora, terapeutičke mete u kognitivnim poremećajima: funkcionalne studije in vitro i in vivo." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:182232

Baković, P. (2015). 'Molekularna farmakologija serotoninskog 5-HT6 receptora, terapeutičke mete u kognitivnim poremećajima: funkcionalne studije in vitro i in vivo', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:182232

Baković P. Molekularna farmakologija serotoninskog 5-HT6 receptora, terapeutičke mete u kognitivnim poremećajima: funkcionalne studije in vitro i in vivo [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:182232

P. Baković, "Molekularna farmakologija serotoninskog 5-HT6 receptora, terapeutičke mete u kognitivnim poremećajima: funkcionalne studije in vitro i in vivo", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:182232

Metadata
TitleMolekularna farmakologija serotoninskog 5-HT6 receptora, terapeutičke mete u kognitivnim poremećajima: funkcionalne studije in vitro i in vivo
Title (english)Molecular pharmacology of the serotonin 5-HT6 receptor, a therapeutic target in cognitive disorders: functional studies in vitro and in vivo
AuthorPetra Baković
MentorVladimir Mrša (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2015-07-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
During this training, we studied interaction of 5-HT6 receptor with β-arrestins and downstream signalling of 5-HT6R, on ERK and mTOR signalling pathway. We provided first evidence of interaction of 5-HT6R with β-arrestins 2 and demonstrated that WAY-208 466 and 5-HT act as agonists on this recruitment, SB-271 act as antagonist and SGS-518 act as inverse agonist. AlphaScreen results from NG 108-15 lysates silenced for β-arrestin 2 revealed decrease in quantity of phosphorylated ERK, leading to conclusion that 5-HT6R could trigger ERK signalling pathway directly through its interactions with β-arrestin 2. We couldn’t obtain any results demonstrating involment of Nf1 on ERK signalling pathway. However we demonstrated involvement of Nf1 in mTOR signalling pathway by westren blot analysis on brain lysates and immunohistochemistry studies on brain sections.
Abstract (french)
Durant ce stage, nous avons étudié l'interaction de R5-HT6 avec les ß-arrestins et leur rôle sur la signalisation de R5-HT6, sur les voies ERK et mTOR. Nous avons montré pour la première fois une intéraction spécifique entre le R5-HT6R et les arrestin 2 et démontré que le WAY 208466 et la 5-HT en favorisant le recrutement de la arrestin 2 agissent comme des agonistes, alors que le SB271 agit comme un antagoniste et le SGS-518 comme un agoniste inverse. La sous-expression des ß-arrestin 2 induit une diminution de la phosphorylation de ERK induite par l'activation du récepteur et mesurée par dosage AlphaScreen, ce qui suggère que la stimulation de ERK implique l'intéraction des ß-arrestin 2. Nous n'avons pas réussi à mettre en évidence un rôle de Nf1 sur l'activation de la voie ERK induite par les agonistes. Par contre, nous montrons qu'une sous expression de Nf1 chez des souris module la voie mTOR aussi bien sur des lysats de tissus cérébraux que sur coupes de cerveau.
Keywords
Languageenglish
URN:NBNurn:nbn:hr:159:182232
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 10:01:13