prikaz prve stranice dokumenta Rekombinogenost palidromskih sekvencija u genomu odabranih mutanata kvasaca Saccharomyces cerevisiae
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master's thesis
Rekombinogenost palidromskih sekvencija u genomu odabranih mutanata kvasaca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:751376
Lončar, Ana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Lončar, A. (2014). Rekombinogenost palidromskih sekvencija u genomu odabranih mutanata kvasaca Saccharomyces cerevisiae (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:751376

Lončar, Ana. "Rekombinogenost palidromskih sekvencija u genomu odabranih mutanata kvasaca Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:751376

Lončar, Ana. "Rekombinogenost palidromskih sekvencija u genomu odabranih mutanata kvasaca Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:751376

Lončar, A. (2014). 'Rekombinogenost palidromskih sekvencija u genomu odabranih mutanata kvasaca Saccharomyces cerevisiae', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:751376

Lončar A. Rekombinogenost palidromskih sekvencija u genomu odabranih mutanata kvasaca Saccharomyces cerevisiae [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:751376

A. Lončar, "Rekombinogenost palidromskih sekvencija u genomu odabranih mutanata kvasaca Saccharomyces cerevisiae", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:751376

Metadata
TitleRekombinogenost palidromskih sekvencija u genomu odabranih mutanata kvasaca Saccharomyces cerevisiae
Title (english)Recombinogenicity of palidndromic sequences in selected mutants of yeast Saccharomyces cerevisiae
AuthorAna Lončar
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2014-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Palindromski sljedovi nukleotida izvor su genetičke nestabilnosti u svim organizmima jer u molekuli DNA formiraju sekundarne strukture (kruciforme i ukosnice) koje se in vivo prevode u dvolančani lom. Međutim, još nije identificiran kvaščev protein koji cijepa kruciforme u genomu kvasca Saccharomyces cerevisiae. U ovom radu korišten je eksperimentalni sustav u kojem se promjena rekombinogenosti palindroma očituje lako uočljivom promjenom boje kolonije, a istražen je utjecaj nekoliko rezolvaza i strukturno-specifičnih endonukleaza koje bi mogle cijepati kruciform. Ustanovljeno je da nedostatak rezolvaze Mus81 ili Yen1 kao i strukturno-specifične endonukleaze Pso2 koja cijepa ukosnice ne utječe na stabilnost 150 pb dugačkog palindroma u genomu kvasca. Ovaj rezultat mogao bi biti posljedica redundantne uloge ovih proteina te je moguće da se njihov utjecaj na stabilnost palindroma može primijetiti tek u višestrukom mutantu.
Abstract (english)
Palindromic sequences are the source of genetic instability in all living organisms. They form secondary structures in DNA (cruciforms and hairpins) which are processed into a double-strand break in vivo. However, the yeast protein that cleaves cruciforms in Saccharomyces cerevisiae genome has not yet been identified. In this research, several resolvases and structure specific endonucleases that could cleave cruciform were tested using the yeast experimental system in which the change of palindrome recombinogenicity is easily observed via colony color change. The absence of Mus81 or Yen1 resolvase, as well as the absence of a structure specific endonuclease Pso2, does not affect the stability of a 150 bp palindrome in the yeast genome. This finding could be a consequence of the overlapping roles of Mus81 and Yen1 proteins indicating that the effect on a palindrome stability could be observed in a double or a triple mutant.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:751376
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 11:17:20