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master's thesis
Višestupanjska enzimska sinteza nukleotidno-aktiviranih šećera
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:603215
Horvat, Doroteja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Horvat, D. (2014). Višestupanjska enzimska sinteza nukleotidno-aktiviranih šećera (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:603215

Horvat, Doroteja. "Višestupanjska enzimska sinteza nukleotidno-aktiviranih šećera." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:603215

Horvat, Doroteja. "Višestupanjska enzimska sinteza nukleotidno-aktiviranih šećera." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:603215

Horvat, D. (2014). 'Višestupanjska enzimska sinteza nukleotidno-aktiviranih šećera', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:603215

Horvat D. Višestupanjska enzimska sinteza nukleotidno-aktiviranih šećera [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 December 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:603215

D. Horvat, "Višestupanjska enzimska sinteza nukleotidno-aktiviranih šećera", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:603215

Metadata
TitleVišestupanjska enzimska sinteza nukleotidno-aktiviranih šećera
Title (english)Multistep enzymatic synthesis of nucleotide-activated sugars
AuthorDoroteja Horvat
MentorAnita Slavica (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2014-10-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
UDP-glukoza sintetizirana je četverostupanjskom reakcijom i dalje, kao supstrat, korištena u proizvodnji UDP-glukuronske kiseline. Označavanje UDP-glukoze izotopima nije imalo utjecaja na ovu reakciju i slični prinosi proizvoda dobiven su s neoznačenim i označenim supstratom. Iako se UDP-glukoza proizvela u trećoj reakciji, provedena je četverostupanjska reakcija. Četvrta reakcija u nizu je provedena jer pirofosfat utječe na prinos UDP-glukoze. Uklanjanje pirofosfata pomaknulo je ravnotežu reakcije prema sintezi UDP-glukoze. Sinteza UDP-glukoze bila je vrlo učinkovita uz prinos proizvoda između 86-99%. Konverzija UDP-glukoze u UDP-glukuronsku kiselinu pomoću humane UDP-glukoza dehidrogenaze provedena je uz prinos od oko 100%. Proizvedena UDP-glukuronska kiselina je pročišćena kao amonijeva sol te kao natrijeva sol. Obje soli korištene su u reakciji koju katalizira UDP-apioza/ksiloza sintaza (AXS). U reakciji s amonijevom solju manje UDP-apioze je nastalo nego u reakciji s natrijevom solju. AXS je jednako koristila sve testirane supstrate, neoznačene i označene UDP-glukuronske kiseline.
Abstract (english)
Four-step synthesis of UDP-Glc was performed and synthesized UDP-Glc was used as substrate in production of UDP-GlcUA. In the synthesis of UDP-Glc, isotope-labeling did not have any influence on the reaction and similar yields were obtained with unlabeled and labeled UDP-Glc. Although UDP-Glc was produced in the 3rd reaction, four-step reaction was performed. Since pyrophosphate affects yield of produced UDP-Glc, 4threaction was performed. Removal of a pyrophosphate dragged equilibrium toward UDP-Glc synthesis. Synthesis of UDP-Glc was very successful with theoretical yield between 86-99%. Conversion of UDP-Glc into UDP-GlcUA by human UDP-glucose dehydrogenase was performed with yield of approximately 100%. Produced UDP-GlcUA was purified as ammonium salt and exchanged into sodium salt. Both salts were used for reaction catalyzed by UDP-apiose/xylose synthase (AXS). In AXS catalyzed reaction with ammonium salt less UDP-Api was formed than in the reaction with sodium salt. AXS accepted all substrates, unlabeled and labeled UDP-GlcUA.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:603215
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 12:40:49