prikaz prve stranice dokumenta Primjena različitih metoda ekstrakcije bioaktivnih komponenta prisutnih u cvijetu bazge
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master's thesis
Primjena različitih metoda ekstrakcije bioaktivnih komponenta prisutnih u cvijetu bazge
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:824608
Mrmić, Mateja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Organic Chemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Mrmić, M. (2015). Primjena različitih metoda ekstrakcije bioaktivnih komponenta prisutnih u cvijetu bazge (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:824608

Mrmić, Mateja. "Primjena različitih metoda ekstrakcije bioaktivnih komponenta prisutnih u cvijetu bazge." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:824608

Mrmić, Mateja. "Primjena različitih metoda ekstrakcije bioaktivnih komponenta prisutnih u cvijetu bazge." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:824608

Mrmić, M. (2015). 'Primjena različitih metoda ekstrakcije bioaktivnih komponenta prisutnih u cvijetu bazge', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:824608

Mrmić M. Primjena različitih metoda ekstrakcije bioaktivnih komponenta prisutnih u cvijetu bazge [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:159:824608

M. Mrmić, "Primjena različitih metoda ekstrakcije bioaktivnih komponenta prisutnih u cvijetu bazge", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:824608

Metadata
TitlePrimjena različitih metoda ekstrakcije bioaktivnih komponenta prisutnih u cvijetu bazge
Title (english)Application of various extraction methods for bioactive components in elderflower
AuthorMateja Mrmić
MentorJasmina Lapić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Organic Chemistry)
Zagreb
Defense date and country2015-07-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Bazga (Sambucus nigra L.) je biljna vrsta koja pripada porodici Caprifoliaceae. Vrlo dobro uspijeva na našim područjima, široko je rasprostranjena i često upotrebljavana u prehrambenoj industriji i farmakologiji. Za potrebe ovog diplomskog rada, iz uoraka sušenog cvijeta bazge, provedena je izolacija bioaktivnih komponenata. Pri postupku izolacije, korištene su sljedeće metode: destilacija vodenom parom, kiselinsko–bazna ekstrakcija (dva različita otapala – diklormetan, etil–acetat), klasična ekstrakcija: grijanje u povrat, te ekstrakcija po Soxhletu. Lakohlapive komponente dobivene destilacijom vodenom parom, identificirane su pomoću tankoslojne kromatografije (TLC) i FTIR–spektroskopije, pri čemu je dokazana prisutnost dominantnih lakohlapivih sastavnica. Ekstrakti dobiveni metodama klasične ekstrakcije grijanjem u povrat i ekstrakcije po Soxhletu, korišteni su za određivanje koncentracije ukupnih fenola u promatranom uorku cvijeta bazge, kao i dijela pojedinih bioaktivnih komponenata. Masena koncentracija ukupnih fenola u etanolnom ekstraktu REF80, iznosila je 35,54 mg GAE/100 g bazge, dok je u ekstraktu SMB80, 13,51 mg GAE/100 g bazge.
Abstract (english)
Elder (Sambucus nigra L.) is a plant species that belongs to the family Caprifoliaceae. Widespread and often used in the food industry and pharmacology, elder is very known in our country. For the purpose of this thesis, from samples of dried elderflower, isolation of bioactive compounds was conducted. In the isolation procedure, the following methods were used: steam distillation, acid–base extraction (two different solvents – dichloromethane, ethyl–acetate), the classical extraction: heating to reflux, and Soxhlet extraction. Volatile components obtained by steam distillation, were identified by thin layer chromatography (TLC) and FTIR–spectroscopy, where the presence of the dominant volatile components was proven. Extracts obtained by classical extraction heated to reflux and Soxhlet extraction, were used to determine the concentration of total phenolics in the observed elderflower sample, as a part of certain bioactive compounds. Mass concentration of total phenolics in the ethanol extract REF80 was 35,54 mg GAE/100 g elder, while in the extract SMB80 was 13,51 mg GAE/100 g elder.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:824608
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-02 13:38:08