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master's thesis
Bioaktivni sastav i biološki učinak ekstrakata mente, ružmarina i đumbira na stanice raka grkljana i pluća
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:614944
Lisičar, Marina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Lisičar, M. (2015). Bioaktivni sastav i biološki učinak ekstrakata mente, ružmarina i đumbira na stanice raka grkljana i pluća (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:614944

Lisičar, Marina. "Bioaktivni sastav i biološki učinak ekstrakata mente, ružmarina i đumbira na stanice raka grkljana i pluća." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:614944

Lisičar, Marina. "Bioaktivni sastav i biološki učinak ekstrakata mente, ružmarina i đumbira na stanice raka grkljana i pluća." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:614944

Lisičar, M. (2015). 'Bioaktivni sastav i biološki učinak ekstrakata mente, ružmarina i đumbira na stanice raka grkljana i pluća', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:614944

Lisičar M. Bioaktivni sastav i biološki učinak ekstrakata mente, ružmarina i đumbira na stanice raka grkljana i pluća [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:614944

M. Lisičar, "Bioaktivni sastav i biološki učinak ekstrakata mente, ružmarina i đumbira na stanice raka grkljana i pluća", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:614944

Metadata
TitleBioaktivni sastav i biološki učinak ekstrakata mente, ružmarina i đumbira na stanice raka grkljana i pluća
Title (english)Bioactive composition and biological effect of mint, rosemary and ginger extracts on the larynx and lung cancer cells
AuthorMarina Lisičar
MentorKsenija Durgo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2015-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Biljne se vrste, posebno menta, ružmarin i đumbir zbog kemijskog sastava prisutnih spojeva i njihovog velikog antioksidacijskog potencijala već dugi niz godina tradicionalno koriste u liječenju različitih bolesti. Ovim je radom provedena detaljna karakterizacija bioaktivnih spojeva (polifenola, pigmenata, polisaharida) navedenih biljnih vrsta i njihovih različito pripremljenih ekstrakata. Također, ispitivani su i biološki učinci na stanice raka grkljana HEp2 i raka pluća A549 stanice. Najveći su udjeli polifenolnih i pigmentnih spojeva određeni u uzorcima mente, koji su pokazali i najveći antioksidacijski kapacitet. Đumbir je bio značajno siromašan ovim spojevima, uz iznimku najvećeg udjela polisaharida. Ekstrakcija biljnih sirovina vodom temperature 80 °C tijekom 15 minuta rezultirala je većim udjelom polifenolnih spojeva u ekstraktima u odnosu na istu provedenu vodom temperature 100 °C tijekom 30 minuta. Ekstrakti mente pokazali su citotoksičnost na obje stanične linije te prooksidativno djelovanje i indukciju lipidne peroksidacije na stanicama A549. Ekstrakti ružmarina djeluju prooksidativno i citotoksično na obje stanične linije te induciraju lipidnu peroksidaciju u stanicama A549. Ekstrakti đumbira štite stanice HEp2 i A549 od slobodnih radikala i oštećenja lipida.
Abstract (english)
Plants, especially mint, rosemary and ginger are traditionally used for disease treatment because of their chemical composition and antioxidant capacity. In this thesis, detailed characterization of bioactive compounds (polyphenols, pigments, polysaccharides) in selected plant materials and their different extracts was investigated. Biological effects of these extracts on the larynx cancer cells HEp2 and lung cancer cells A549 were also evaluated. Mint sample had the highest amounts of polyphenols and pigments so the highest antioxidant capacity. Despite lowest amounts of the polyphenols and pigments, ginger sample had the highest level of polysaccharides. Extraction of plant raw materials with water at 80 °C for 15 minutes resulted in a greater share of polyphenolic compounds extracts when compared to the extraction at a temperature of 100 °C for 30 minutes. Cytotoxic effect was seen on the HEp2 cells and prooxidative effect with induction of the lipid peroxidation was observed in the A549 cells incubated with mint extract. Rosemary extract showed cytotoxic and prooxidative effect on the HEp2 and A549, and induction of the lipid peroxidation on the A549. Ginger extract decreased the concentration of free radicals and also protect lipids of HEp2 and A549 cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:614944
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-03 07:45:29