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master's thesis
Kinetičko - procesna analiza kontinuiranog 5 - stupanjskog procesa biosinteze PHB / poli-(3-hidroksibutirata) / primjenom elektronske mikroskopije
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:707664
Vadlja, Denis
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vadlja, D. (2015). Kinetičko - procesna analiza kontinuiranog 5 - stupanjskog procesa biosinteze PHB / poli-(3-hidroksibutirata) / primjenom elektronske mikroskopije (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:707664

Vadlja, Denis. "Kinetičko - procesna analiza kontinuiranog 5 - stupanjskog procesa biosinteze PHB / poli-(3-hidroksibutirata) / primjenom elektronske mikroskopije." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:707664

Vadlja, Denis. "Kinetičko - procesna analiza kontinuiranog 5 - stupanjskog procesa biosinteze PHB / poli-(3-hidroksibutirata) / primjenom elektronske mikroskopije." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:707664

Vadlja, D. (2015). 'Kinetičko - procesna analiza kontinuiranog 5 - stupanjskog procesa biosinteze PHB / poli-(3-hidroksibutirata) / primjenom elektronske mikroskopije', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 September 2024, https://urn.nsk.hr/urn:nbn:hr:159:707664

Vadlja D. Kinetičko - procesna analiza kontinuiranog 5 - stupanjskog procesa biosinteze PHB / poli-(3-hidroksibutirata) / primjenom elektronske mikroskopije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 September 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:707664

D. Vadlja, "Kinetičko - procesna analiza kontinuiranog 5 - stupanjskog procesa biosinteze PHB / poli-(3-hidroksibutirata) / primjenom elektronske mikroskopije", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:707664

Metadata
TitleKinetičko - procesna analiza kontinuiranog 5 - stupanjskog procesa biosinteze PHB / poli-(3-hidroksibutirata) / primjenom elektronske mikroskopije
Title (english)Analysis of process kinetic of PHB biosynthesis in continuous production using the cascade of 5 biorectors and electron microscopy
AuthorDenis Vadlja
MentorPredrag Horvat (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2015-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom istraživanju analiziran je peterostupanjski reaktorski sustav za kontinuiranu proizvodnju PHB-a. Korištena je skenirajuća transmisijska elektronska mikroskopija (STEM). Programski paket ImageJ primijenjen je za analizu veličine stanica i unutarstaničnih poli-(3-hidroksibutirat) (PHB) granula. Primijenjena je tehnika digitalne analize STEM snimaka uz mjerenje tlocrtne površine stanica i PHB granula (pod pretpostavkom da između površine na slici i realnog volumena objekta postoji direktna proporcionalnost). Specifične brzine rasta (μ) i specifične brzine nastajanja PHB-a (qP) za kulturu Cupriavidus necator DSM 545 određene su gravimetrijski i pomoću odnosa tlocrtnih površina stanica i PHB granula za svih pet reaktora. Dobiveni rezultati uspoređeni su međusobno. Na temelju dobivenih rezultata, zaključeno je da se ImageJ program može uspješno koristiti za određivanje veličine stanica i granula mjerenjem tlocrtne površine istih na STEM snimkama. Specifična brzina rasta (μ) određena mjerenjem tlocrtne površine stanica slaže se sa specifičnom brzinom rasta (μ) određenom gravimetrijski.
Abstract (english)
In this study, a 5-step reactor system for the production of PHB was analyzed. Scanning transmission electron microscopy (STEM) and ImageJ software package were used to anaylize the cell size and the size of intracellular poly-3-hydroxybutyrate (PHB) granules. Digital imaging technique (analysis) of the STEM images was applied for the measuring of cells and PHB-granula areas (assuming that there is a direct proportionality between the image area and the related real object volume). The specific growth rate (μ) and the specific PHB productivity rate (qp) for Cupriavidus necator DSM 545 culture were determined by gravimetric and by digital imaging technique analysis for all five reactors in cascade. The results were compared mutually. Based on the obtained results, it is evident that the ImageJ software package can be successfully used for the determination of cells and PHB-granula areas present in the STEM images. The specific growth rate (μ) determined by digital imaging technique analysis matches very well with those determined by gravimetric method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:707664
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-03 09:31:17