prikaz prve stranice dokumenta Optimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina
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master's thesis
Optimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:185437
Tuđan, Maja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for Fermentation and Yeast Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Tuđan, M. (2015). Optimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:185437

Tuđan, Maja. "Optimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:185437

Tuđan, Maja. "Optimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:185437

Tuđan, M. (2015). 'Optimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:185437

Tuđan M. Optimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:185437

M. Tuđan, "Optimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:185437

Metadata
TitleOptimiranje sastava podloge za rast kvasca Scheffersomyces stipitis i proizvodnju S-adenozil-L-metionina
Title (english)Optimization of medium composition for growth yeast Scheffersomyces stipitis and production of S-adenosyl-L-methionine
AuthorMaja Tuđan
MentorDamir Stanzer (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Engineering)
(Laboratory for Fermentation and Yeast Technology)
Zagreb
Defense date and country2015-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
S-adenozil-L-metionin (SAMe) je biološki važna molekula, sudionik mnogih biokemijskih reakcija kod sisavaca. Poremećaji u njegovom metabolizmu povezani su s bolestima jetre te raznim neurološkim poremećajima. Budući da se SAMe koristi pri liječenju ovih oboljenja, važno je pronaći učinkovite načine njegove proizvodnje. Kvasac Scheffesomyces stipitis koristi se za proizvodnju etanola i mikrobne biomase, te za proizvodnju heterolognih proteina. U ovom radu uzgajana je biomasa soja M12 te divljeg soja kvasca S. stipitis na različitim podlogama s ciljem optimiranja proizvodnje SAMe. Divlji soj kvasca brže raste u odnosu na soj M12 u svim istraživanim podlogama, ali soj M12 proizvodi veće količine SAMe. Kod soja M12 nakon 48 sati u O-podlozi s dodatkom glicerola izmjerena je količina SAMe od 24,5 mgg-1 s.tv., dok je kod divljeg soja ona iznosila 12,2 mgg-1 s.tv. Također, uočeno je i da smanjenje volumena podloge, tj. povećanje aeracije povećava udio SAMe u biomasi soja M12 kvasca S. stipitis.
Abstract (english)
S-adenosyl-L-methionine (SAMe) is a biologicaly important molecule because is involved in several biochemical reactions of mammals. Abnormalities in its metabolism are recognized in liver disease and various neurological disorders. Since SAMe is used in the treatment of such conditions, it is important to find effective ways of its production. Yeast Scheffesomyces stipitis is used for the production of ethanol and microbial biomass and for production of heterologous proteins. In this work, wild S. stipitis strain and M12 strain were grown on different media with the aim of optimize the production of SAMe. The wild strain grows faster in comparison to the M12 strain in all tested media but the M12 strain produces higher amounts of SAMe. The content of SAM in the M12 strain was 24.5 mgg-1 dry weight while the wild strain was 12.2 mgg-1 dry weight after 48 hour of cultivation in O-medium with addition of glycerol. Also, it was observed that volume reducing of the medium (ie. increasing aeration) increases the amount of SAMe in the biomass of S. stipitis M12 strain.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:185437
Study programmeTitle: Food Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-03 10:04:31