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undergraduate thesis
Proizvodnja rekombinantnog proteina u CHO staničnoj liniji
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:346190
Bojanić, Karla
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bojanić, K. (2016). Proizvodnja rekombinantnog proteina u CHO staničnoj liniji (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:346190

Bojanić, Karla. "Proizvodnja rekombinantnog proteina u CHO staničnoj liniji." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:346190

Bojanić, Karla. "Proizvodnja rekombinantnog proteina u CHO staničnoj liniji." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:346190

Bojanić, K. (2016). 'Proizvodnja rekombinantnog proteina u CHO staničnoj liniji', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 06 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:346190

Bojanić K. Proizvodnja rekombinantnog proteina u CHO staničnoj liniji [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 May 06] Available at: https://urn.nsk.hr/urn:nbn:hr:159:346190

K. Bojanić, "Proizvodnja rekombinantnog proteina u CHO staničnoj liniji", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:346190

Metadata
TitleProizvodnja rekombinantnog proteina u CHO staničnoj liniji
Title (english)The production of recombinant proteins in CHO cell line
AuthorKarla Bojanić
MentorIgor Slivac (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2016-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Proizvodnja rekombinantnih proteina u svrhu istraživanja i komercijalne upotrebe počima uspostavljanjem stanične linije. CHO stanična linija pogodna je za korištenje u tehologiji stanica jer se može genetički modificirati, uzgajati u suspenziji te raditi scale-up. Stanice mogu rasti u kemijskim definiranim medijima bez seruma i proteinskih hidrolizata. Cilj ovog rada je bio ispitati učinak različitih sastava medija za uzgoj na rast stanične linije, utrošak glukoze te proizvodnju proteina. Medij s 5 % seruma i 4% sojinog hidrolizata pokazao se kao najbolj za rast i prinos proteina, medij s 5% seruma bez sojinog hidrolizata je pokazao sporiji rast,dok je u mediju sa 1% seruma i 4% sojinog hidrolizata najsporiji rast što pokazuje da sojin hidrolizat nije dovoljno učinkovita zamjena za serum.
Abstract (english)
The production of recombinant proteins for research and commercial use begins by establishing a cell line. CHO cell line is suitable for use in technology of animal cells because cells can be genetically modified , grown in suspension , and the scale-up work. The cells may be grown in chemically defined medium without serum and protein hydrolysates . The purpose of this work was to examine the effect of different composition of culture medium on the growth of cell lines, the consumption of glucose and protein production. It was shown that the best medium for cells growth and protein yield was obtain with 5 % serum and 4 % soy hydrolyzate, medium with 5 % serum without soy hydrolyzate showed slower growth , while in the medium with 1 % serum and 4 % soy hydrolyzate resulted in the lowest cell growth and was shown that soy hydrolyzate is not sufficiently effective substitute for serum.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:346190
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-21 12:16:41