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master's thesis
Proizvodnja lipida sa kvascem Trichosporon oleaginosus na glukozi i ksilozi uz limitaciju rasta izvorom dušika
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. urn:nbn:hr:159:692164
Lisičar, Josipa
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Lisičar, J. (2013). Proizvodnja lipida sa kvascem Trichosporon oleaginosus na glukozi i ksilozi uz limitaciju rasta izvorom dušika (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:692164

Lisičar, Josipa. "Proizvodnja lipida sa kvascem Trichosporon oleaginosus na glukozi i ksilozi uz limitaciju rasta izvorom dušika." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:692164

Lisičar, Josipa. "Proizvodnja lipida sa kvascem Trichosporon oleaginosus na glukozi i ksilozi uz limitaciju rasta izvorom dušika." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:692164

Lisičar, J. (2013). 'Proizvodnja lipida sa kvascem Trichosporon oleaginosus na glukozi i ksilozi uz limitaciju rasta izvorom dušika', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:692164

Lisičar J. Proizvodnja lipida sa kvascem Trichosporon oleaginosus na glukozi i ksilozi uz limitaciju rasta izvorom dušika [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2013 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:692164

J. Lisičar, "Proizvodnja lipida sa kvascem Trichosporon oleaginosus na glukozi i ksilozi uz limitaciju rasta izvorom dušika", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:159:692164

Metadata
TitleProizvodnja lipida sa kvascem Trichosporon oleaginosus na glukozi i ksilozi uz limitaciju rasta izvorom dušika
Title (english)Lipid production by yeast Trichosporon oleaginosus on glucose and xylose under nitrogen limitation of growth
AuthorJosipa Lisičar
MentorMirela Ivančić - Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2013-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Nakupljanje lipida u oleaginoznim mikroorganizmima započinje kada je jedan nutrijent u hranjivoj podlozi utrošen (najčešćeg izvor dušika), a izvor ugljika, koji je u suvišku, troši se za sintezu triacilglicerola. U ovom istraživanju proučavana je proizvodnja lipida sa kvascem Trichosporon oleaginosus na hranjivim podlogama s glukozom i/ili ksilozom kao izvorom ugljika uz različite omjere masa izvora ugljika i dušika (C/N). Najveći udjela lipida postignut je uz omjer masa C/N od 45 g g-1. Ksiloza se pokazala pogodnijim supstratom za proizvodnju lipida nego glukoza. Tijekom rasta u podlozi s glukozom i ksilozom kvasac redoslijedno troši izvore ugljika. Kada koncentracija glukoze padne ispod 5 g L-1, kvasac počinje rasti na ksilozi. Tijekom šaržnog uzgoja s pritokom supstrata postignut je vrlo visoki udjel lipida u stanici od 64,84 %, dok je produktivnost procesa iznosila 0,227 g L-1 h-1.
Abstract (english)
Lipid accumulation in oleaginous microorganisams begins when one nutrient from the medium (usually nitrogen) is exhausted but an excess of carbon is still assimilated by the cells and is converted into triacylglycerols. Production of lipid by yeast Trichosporon oleaginosus on the medium contained glucose and/or xylose as the carbon source was studied in this research. Optimum C/N ratio for lipid accumulation was 45 g g-1 with glucose as carbon source. Maximum lipid accumulation occurred in batch culture with xylose. In the medium containing glucose and xylose microogranism showed sequential utilization of substrates. When the concentration of glucose decreased below than 5 g L-1, yeast started to spend xylose as the source of carbon. Maximum lipid accumulation occurred in fed-batch culture with glucose reaching a level of 64.34 % of the biomass and productivity of 0.227 g L-1 h-1.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:692164
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-04 10:45:56