prikaz prve stranice dokumenta Utjecaj promjene temperature na selektivnost inhibicije bambuterolom i stereoselektivnost kolinesteraza
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master's thesis
Utjecaj promjene temperature na selektivnost inhibicije bambuterolom i stereoselektivnost kolinesteraza
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. urn:nbn:hr:159:769147
Dropulić, Tihana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Dropulić, T. (2013). Utjecaj promjene temperature na selektivnost inhibicije bambuterolom i stereoselektivnost kolinesteraza (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:769147

Dropulić, Tihana. "Utjecaj promjene temperature na selektivnost inhibicije bambuterolom i stereoselektivnost kolinesteraza." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:769147

Dropulić, Tihana. "Utjecaj promjene temperature na selektivnost inhibicije bambuterolom i stereoselektivnost kolinesteraza." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:769147

Dropulić, T. (2013). 'Utjecaj promjene temperature na selektivnost inhibicije bambuterolom i stereoselektivnost kolinesteraza', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 September 2024, https://urn.nsk.hr/urn:nbn:hr:159:769147

Dropulić T. Utjecaj promjene temperature na selektivnost inhibicije bambuterolom i stereoselektivnost kolinesteraza [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2013 [cited 2024 September 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:769147

T. Dropulić, "Utjecaj promjene temperature na selektivnost inhibicije bambuterolom i stereoselektivnost kolinesteraza", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:159:769147

Metadata
TitleUtjecaj promjene temperature na selektivnost inhibicije bambuterolom i stereoselektivnost kolinesteraza
Title (english)The effect of temperature on bambuterol inhibition selectivity and cholinesterase stereoselectivity
AuthorTihana Dropulić
MentorVišnja Gaurina Srček (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2013-01-31, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Bambuterol je ireverzibilni inhibitor kolinesteraza koji selektivno inhibira butirilkolinesterazu (BChE; EC 3.1.1.8). Za inhibiciju acetilkolinesteraze (AChE; EC 3.1.1.7) i BChE s racemičnim, (R)- i (S)-bambuterolom određene su sveukupne konstante brzine inhibicije (ki) pri 12, 20, 25, 30 i 37 °C. Selektivnost inhibicije je izražena kao omjer ki konstanti određenih za inhibiciju AChE i BChE s racemičnim bambuterolom, dok je stereoselektivnost BChE određena kao omjer ki konstanti za (R)- i (S)-bambuterol. Za BChE je pokazano da brzina inhibicije raste linearno s porastom temperature do 30 °C dok je brzina inhibicije pri 37 °C jednaka onoj na 25 °C. Utvrđena ovisnost brzine inhibicije o temperaturi ukazuje da se kod 37 °C mijenja odnos konstanti brzina koje opisuju pojedine reakcije složenog mehanizma inhibicije BChE bambuterolom definiranog ki konstantom, u odnosu na temperature do 30 °C. Porastom temperature selektivnost inhibicije bambuterolom opada, dok se stereoselektivnost BChE prema enantiomerima bambuterola s promjenom temperature ne mijenja.
Abstract (english)
Bambuterol is an irreversible cholinesterase inhibitor that selectively inhibits butyrylcholinesterase (BChE, EC 3.1.1.8). The overall inhibition rate constant (ki) for the inhibition of acetylcholinesterase (AChE; EC 3.1.1.7) and BChE with racemic, (R) - or (S)-bambuterol were determined at 12, 20, 25, 30 and 37 °C. The selectivity of inhibition was expressed as the ratio of ki constants for the inhibition of AChE and BChE with racemic bambuterol, while BChE stereoselectivity was defined as the ratio of ki constants for (R)- and (S)-bambuterol. The inhibition rate of BChE linearly increased at temperatures up to 30 °C, whereas at 37 °C the inhibition rate decreased and was similar to the inhibition rate determined at 25 °C. An increase in temperature affects the ratio of constants describing the particular reactions in the mechanism of BChE inhibition by bambuterol. The ratio of particular constants changed at 37 °C compared to that at temperatures below 30°C. Increasing the temperature caused a decrease in the selectivity of cholinesterase inhibition by bambuterol, while the stereoselectivity of BChE toward bambuterol enantiomers did not change with temperature.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:769147
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-04 12:15:01