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master's thesis
Hidrotermalna i biokatalitička konverzija lignoceluloznih obnovljivih sirovina
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. urn:nbn:hr:159:225057
Vitez, Ivan
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vitez, I. (2013). Hidrotermalna i biokatalitička konverzija lignoceluloznih obnovljivih sirovina (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:225057

Vitez, Ivan. "Hidrotermalna i biokatalitička konverzija lignoceluloznih obnovljivih sirovina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:225057

Vitez, Ivan. "Hidrotermalna i biokatalitička konverzija lignoceluloznih obnovljivih sirovina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:225057

Vitez, I. (2013). 'Hidrotermalna i biokatalitička konverzija lignoceluloznih obnovljivih sirovina', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 16 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:225057

Vitez I. Hidrotermalna i biokatalitička konverzija lignoceluloznih obnovljivih sirovina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2013 [cited 2024 November 16] Available at: https://urn.nsk.hr/urn:nbn:hr:159:225057

I. Vitez, "Hidrotermalna i biokatalitička konverzija lignoceluloznih obnovljivih sirovina", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:159:225057

Metadata
TitleHidrotermalna i biokatalitička konverzija lignoceluloznih obnovljivih sirovina
Title (english)Hydrothermal and biocatalytic conversion of lignocellulosic renewable resources
AuthorIvan Vitez
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
Zagreb
Defense date and country2013-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada je bio optimizirati proces predobrade bukovog drveta kako bi se odstranila hemiceluloza i/ili lignin od celuloznih vlakana, čineći tako vlakna dostupnijim celulolitičkim enzimima. Dva su postupka korištena za ekstrakciju drveta: "Organosolv" i "Liquid Hot Water". Postupci su istraživani pod različitim temperaturama, omjerima između tekuće i krute faze, te međusobno kombinirani. Kao mjera za uspješnost ekstrakcije poslužila je koncentracija glukoze nakon enzimske hidrolize dobivenih vlakana pri koncentraciji od 200 g*L-1, kao i koncentracija potencijalnih inhibitora - furfurala i hidroksimetilfurfurala. Zaključeno je da duže trajanje predobrade drveta u kombinaciji sa većim omjerom tekuće/kruto rezultira većim prinosom glukoze nakon enzimske hidrolize, kao i većim koncentracijama furfurala i hidroksimetilfurfurala u supernatantu nakon ekstrakcije. Nadalje, istraživan je i utjecaj povišenog tlaka na enzimsku hidrolizu kao i stabilizacija Cellic® CTec2 enzima pod povišenim tlakom. Ekonomski potencijal biorafinerija bi se mogao povećati ukoliko bi se postiglo kraće vrijeme enzimske hidrolize pod povišenim tlakom. Rezultati pokazuju da povišeni tlak pozitivno utječe na enzimsku aktivnost a što je uočeno kroz povećanje koncentracije glukoze nakon hidrolize pod povišenim pritiskom.
Abstract (english)
Goal of this study was to optimize pre-treatment conditions for beech wood to remove lignin and/or hemicellulose from cellulosic fibers, thus making them more accessible for cellulolytic enzymes. Focusing on "Organosolv"- and "Liquid Hot Water" extraction, the effect of temperature, extraction time, liquid to solid ratio and the combination of both methods have been investigated. As measurable factors for successful extraction were the glucose concentration after enzymatic hydrolysis of the gained fiber at 200 g*L-1 and concentrations of the potential inhibitors HMF and furfural. It was concluded that longer pretreatment time in combination with higher liquid to solid ratio results in higher glucose yield after enzymatic hydrolysis and higher HMF and furfural concentrations in pretreatment liquor. Furthermore, enzymatic hydrolysis under high pressures, and pressure impact on the stabilization of Cellic® CTec2 enzymes were studied. The economic potential of biorefineries could be considerably improved when hydrolysis time under high pressure will be reduced. The results obtained in these experiments show that high pressure has positive impact on the enzymatic activity and consequently higher sugar concentrations were observed after hydrolysis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:225057
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2017-05-04 13:23:14