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undergraduate thesis
Transformacija kvasca Saccharomyces cerevisiae plazmidom koji sadrži gen YAP1 odgovoran za rezistenciju na inhibitore rasta
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:561226
Jalšić, Lovro
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Jalšić, L. (2016). Transformacija kvasca Saccharomyces cerevisiae plazmidom koji sadrži gen YAP1 odgovoran za rezistenciju na inhibitore rasta (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:561226

Jalšić, Lovro. "Transformacija kvasca Saccharomyces cerevisiae plazmidom koji sadrži gen YAP1 odgovoran za rezistenciju na inhibitore rasta." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:561226

Jalšić, Lovro. "Transformacija kvasca Saccharomyces cerevisiae plazmidom koji sadrži gen YAP1 odgovoran za rezistenciju na inhibitore rasta." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:561226

Jalšić, L. (2016). 'Transformacija kvasca Saccharomyces cerevisiae plazmidom koji sadrži gen YAP1 odgovoran za rezistenciju na inhibitore rasta', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:561226

Jalšić L. Transformacija kvasca Saccharomyces cerevisiae plazmidom koji sadrži gen YAP1 odgovoran za rezistenciju na inhibitore rasta [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:561226

L. Jalšić, "Transformacija kvasca Saccharomyces cerevisiae plazmidom koji sadrži gen YAP1 odgovoran za rezistenciju na inhibitore rasta", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:561226

Metadata
TitleTransformacija kvasca Saccharomyces cerevisiae plazmidom koji sadrži gen YAP1 odgovoran za rezistenciju na inhibitore rasta
Title (english)Transformation of yeast Saccharomyces cerevisiae with plasmid containing growth inhibitor resistance gene YAP1
AuthorLovro Jalšić
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2016-06-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Neki od spojeva koji nastaju kiselinskom predobradom lignoceluloznih materijala inhibiraju rast kvasca Saccharomyces cerevisiae jer povećavaju količinu reaktivnih kisikovih spojeva u stanici. Gen YAP1 kodira za transkripcijski faktor koji u prisutnosti reaktivnih kisikovih spojeva povećava razinu ekspresije mnogih gena čiji proteinski produkti sudjeluju u smanjenju oksidativnog stresa. Cilj ovog rada bio je transformirati odabrane diploidne sojeve kvasca S. cerevisiae, nastale križanjem prirodnih izolata iz vina (E5) i cvijeta kaktusa Opunitha megacantha (2421), plazmidom pSP-YAP1 koji sadrži gen YAP1. Prvo je restrikcijskom analizom provjerena struktura plazmida pSP-YAP1, nakon čega su transfomirani homozigotni diploidni sojevi E5 i 2421 te heterozigotni diploidni soj H2 nastao križanjem haploidnih sojeva E5 i 2421.
Abstract (english)
Some compounds that are by-products of the acidic hydrolysis of a raw lignocellulosic material inhibit the growth of the yeast Saccharomyces cerevisiae due to the accumulation of reactive oxygen species in the cell. YAP1 gene encodes transcription factor that, in the presence of reactive oxygen species, increases the expression of genes that encode proteins involved in the response to oxidative stress. The aim of this work was to transform selected diploid S. cerevisiae strains that were constructed by mating of E5, a natural isolate from wine, and 2421, an isolate from the flower of the cactus Opunitha megacantha, with the plasmid pSP-YAP1 containing the YAP1 gene. First, the structure of the plasmid pSP-YAP1 was confirmed by restriction mapping and then homozygote diploid strains E5 and 2421 and heterozygote diploid H2 strain were transformed successfully.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:561226
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-21 12:26:44