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master's thesis
Kemijska sterilizacija za heterotrofni uzgoj alge Euglena gracilis
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. urn:nbn:hr:159:854491
Crnić, Dijana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Crnić, D. (2013). Kemijska sterilizacija za heterotrofni uzgoj alge Euglena gracilis (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:854491

Crnić, Dijana. "Kemijska sterilizacija za heterotrofni uzgoj alge Euglena gracilis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:854491

Crnić, Dijana. "Kemijska sterilizacija za heterotrofni uzgoj alge Euglena gracilis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:854491

Crnić, D. (2013). 'Kemijska sterilizacija za heterotrofni uzgoj alge Euglena gracilis', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:854491

Crnić D. Kemijska sterilizacija za heterotrofni uzgoj alge Euglena gracilis [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2013 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:854491

D. Crnić, "Kemijska sterilizacija za heterotrofni uzgoj alge Euglena gracilis", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:159:854491

Metadata
TitleKemijska sterilizacija za heterotrofni uzgoj alge Euglena gracilis
Title (english)Chemical sterilization for heterotrophic cultivation of algae Euglena gracilis
AuthorDijana Crnić
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2013-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom istraživanju proučavan je postupak kemijske sterilizacije Hutnerove podloge za heterotrofni uzgoj alge E. gracilis. Vodikov peroksid i antimicin odobreni su kao sredstva za kemijsku sterilizaciju, a kvasac Saccharomyces cerevisiae i bakterija Bacillus subtilis kao predstavnici kontaminanata heterotrofnog uzgoja E. gracilis. Na osnovi rezultata istraživanja dobiveno je da je koncentracija vodikovog peroksida u intervalu od 4,5-7,5 mg/L optimalna za heterotrofni uzgoj E. gracilis pri čemu je uočeno usporavanje rasta kvasca i bakterije. Koncentracija antimicina od 0,2 g/L usporava rast E. gracilis i kontaminanata osobito bakterije B. subtilis a koncentracija od 0,5 g/L u potpunosti zaustavlja heterotrofni uzgoj E. gracilis. Na osnovi rezultata ovog istraživanja vidljivo je da je nužno daljnje istraživanje s ciljem definiranja optimalne koncentracije antimicina za heterotrofni uzgoj E. gracilis.
Abstract (english)
In this research chemical sterilization of Hutner medium for heterotrophic cultivation of E. gracilis was studied. Hydrogen peroxide and antimycin were selected as active compounds for chemical sterilization and yeast Saccharomyces cerevisiae and bacterium Bacillus subtilis as representitive contaminants of E. gracilis cultivation in heterotrophic cultivation. Respecticely on the basis of results obtained in this research it is evident that optimal H2O2 concentration interval for heterotrophic cultivation of E. gracilis is 4,5-7,5 mg/L where notable slow down of yeast and bacterium growth was observed. Antimycin concentracion of 0,2 g/L considerably slow down the growth of E. gracilis and contaminants (especially B. subtilis). However, antimycin concentracion of 0,5 mg /L completely stopped heterotrophic cultivation of E. gracilis. On the basis of this research it is clear that further investigation has to be performed in order to define the optimal antimycin concentracion for heterotrophic cultivation of E. gracilis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:854491
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-05 09:54:00