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master's thesis
Određivanje titra enterovirusa serotipa 71
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:466924
Kajba, Nives
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kajba, N. (2012). Određivanje titra enterovirusa serotipa 71 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:466924

Kajba, Nives. "Određivanje titra enterovirusa serotipa 71." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:466924

Kajba, Nives. "Određivanje titra enterovirusa serotipa 71." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:466924

Kajba, N. (2012). 'Određivanje titra enterovirusa serotipa 71', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:466924

Kajba N. Određivanje titra enterovirusa serotipa 71 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 November 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:466924

N. Kajba, "Određivanje titra enterovirusa serotipa 71", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:466924

Metadata
TitleOdređivanje titra enterovirusa serotipa 71
Title (english)Determination of Enterovirus serotype 71 titer
AuthorNives Kajba
MentorJasna Franekić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-02-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Primjena kultura stanica omogućava dokazivanje viralne replikacije mikroskopski, te godinama osigurava odgovarajući okoliš za detekciju i identifikaciju brojnih humanih viralnih patogena. Cilj ovog istraživanja bila je izolacija i umnažanje EV71 iz kliničkog uzorka cerebrospinalne tekućine bolesnika na RD staničnoj liniji, mikroskopska detekcija EV71 imunoflourescencijskom (IF) metodom i praćenjem citopatogenog učinka (CPE) virusa na RD stanicama, te određivanje titra umnoženog EV71 u mikrotitarskoj pločici određivanjem CPE virusa. Dobiveni rezultati pokazuju da EV71 postiže visoki titar (0,464-1 × 108/mL) kada se uzgaja na odgovarajućoj staničnoj liniji, kao što je RD stanična linija. Identifikacija EV71 potvrđena je primjenom imunoflourescencijske metode.
Abstract (english)
Cell cultures are convenient to examine microscopically for evidence of viral replication, and, for many years, have provided a desirable environment for the detection and identification of many human viral pathogens. The aim of this work was isolation and propagation of Enterovirus 71 from cerebrospinal fluid specimen in RD cell line, microscopic detection of the virus using immunofluorescence (IF) technique and cytopathic effect (CPE) as well viral titer determination by examining the occurrence of CPE in a RD cell monolayer. The obtained results have shown that EV71 reaches high titers (0,464-1 × 108/mL) when grown within appropriate cell line, such as RD cell line. Identification of EV71 was confirmed using the immunofluorescence method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:466924
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-08 10:17:26