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master's thesis
Istraživanje genotoksičnog, antioksidativnog i prooksidativnog učinka kvercetina na ljudskim stanicama karcinoma grkljana (HEp2)
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:331325
Ivelić, Maja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Ivelić, M. (2012). Istraživanje genotoksičnog, antioksidativnog i prooksidativnog učinka kvercetina na ljudskim stanicama karcinoma grkljana (HEp2) (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:331325

Ivelić, Maja. "Istraživanje genotoksičnog, antioksidativnog i prooksidativnog učinka kvercetina na ljudskim stanicama karcinoma grkljana (HEp2)." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:331325

Ivelić, Maja. "Istraživanje genotoksičnog, antioksidativnog i prooksidativnog učinka kvercetina na ljudskim stanicama karcinoma grkljana (HEp2)." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:331325

Ivelić, M. (2012). 'Istraživanje genotoksičnog, antioksidativnog i prooksidativnog učinka kvercetina na ljudskim stanicama karcinoma grkljana (HEp2)', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:331325

Ivelić M. Istraživanje genotoksičnog, antioksidativnog i prooksidativnog učinka kvercetina na ljudskim stanicama karcinoma grkljana (HEp2) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:331325

M. Ivelić, "Istraživanje genotoksičnog, antioksidativnog i prooksidativnog učinka kvercetina na ljudskim stanicama karcinoma grkljana (HEp2)", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:331325

Metadata
TitleIstraživanje genotoksičnog, antioksidativnog i prooksidativnog učinka kvercetina na ljudskim stanicama karcinoma grkljana (HEp2)
Title (english)Research of genotoxic, antioxidative and pooxidative effects of quercetin on human laryngeal carcinoma (HEp2) cells
AuthorMaja Ivelić
MentorKsenija Durgo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Kvercetin, flavonoid koji je u visokoj koncentraciji prisutan u luku, jabukama, grožđu i drugim namirnicama, karakterizira nekoliko bioloških učinaka: antioksidativno djelovanje, protuupalna, antivirusna i antitumorska aktivnost. Cilj ovoga rada je bio ispitati citotoksično djelovanje kvercetina na humanim stanicama karcinoma grkljana (HEp2) i utvrditi koju vrstu stanične smrti izaziva ovaj spoj u širokom rasponu koncentracija (1-3000 µM). Također, na temelju jednolančanih i dvolančanih lomova izazvanih ovim spojem utvrđen je mutageni, prooksidativni i antioksidativni potencijal kvercetina. Citotoksičnost kvercetina je ovisna o koncentraciji i vremenu inkubacije HEp2 stanica, a značajno smanjenje preživljenja stanica zapaženo je kod koncentracije 100 µM i viših koncentracija. Nadalje, izlaganje stanica kvercetinu uzrokuje apoptozu i nekrozu, s tim da je apoptoza bila dominantan način stanične smrti. Oštećenja DNA izazvana kvercetinom ovisila su o koncentraciji i vremenu inkubacije. Rezultati pokazuju da ovaj spoj inhibira oštećenje DNA izazvano vodikovim peroksidom u koncentraciji 20 µM i da u toj koncentraciji sam ne izaziva oštećenja DNA.
Abstract (english)
Quercetin, a flavonoid present in high concentrations in onion, apples, grapes and other food products, is characterized by several biological effects: antioxidant activity, antiinflammatory, antiviral and anticancer activity. The aim of this study was to investigate the cytotoxic effect of quercetin on human laryngeal carcinoma cells (HEp2) and to determine the type of cell death caused by this compound in a wide concentration range (1-3000 µM). Also, its ability to induce/prevent single and double strand DNA breaks was determined. In order to determine its antioxidative nature, inhibition of DNA breaks was determined in the cells previously treated with H2O2. The cytotoxicity of quercetin is dependent on the concentration and time of incubation HEp2 cells, a significant decrease in cell survival was observed at concentrations of 100 µM and higher. The dominant mode of cell death caused with quercetin is apoptosis. It causes DNA breaks time- and concentration-dependent manner. Quercetin inhibits hydrogen peroxide-induced DNA damage at concentration of 20 µM and does not cause DNA damage itself.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:331325
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-08 10:23:51