prikaz prve stranice dokumenta Rekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae
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master's thesis
Rekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:366831
Gardlo, Nikolina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Gardlo, N. (2012). Rekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:366831

Gardlo, Nikolina. "Rekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:366831

Gardlo, Nikolina. "Rekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:366831

Gardlo, N. (2012). 'Rekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 03 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:366831

Gardlo N. Rekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 December 03] Available at: https://urn.nsk.hr/urn:nbn:hr:159:366831

N. Gardlo, "Rekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:366831

Metadata
TitleRekombinogenost pseudopalindroma u genomu kvasca Saccharomyces cerevisiae
Title (english)Recombinogenicity of pseudopalindromes in yeast Saccharomyces cerevisiae
AuthorNikolina Gardlo
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Genomi svih organizama, a posebice viših eukariota, sadrže značajan udio ponovljenih sekvencija. Posebnu skupinu ponovljenih sekvencija čine bliska obrnuta ponavljanja, a nazivaju se palindromi (ili savršeni palindromi) i pseudopalindromi (nesavršeni palindromi). Naime, palindromi su identična obrnuta ponavljanja između kojih nema razmaka, dok ponavljanja u pseudopalindromima nisu identična i/ili između njih postoji razmak od jednog ili više parova baza (pb). Palindromi i pseudopalindromi u molekuli DNA mogu tvoriti sekundarne strukture koje mogu uzrokovati lomove kromosoma što zatim može dovesti do genetičkih bolesti kod ljudi. U ovom radu provedeno je istraživanje utjecaja udaljenost obrnutih ponavljanja unutar pseudopalindroma na njihovu rekombinogenost pri čemu je kao eksperimentalni organizam korišten kvasac Saccharomyces cerevisiae. Dobiveni rezultati pokazuju da umetanje razmaknice duljine 4 pb u centar palindroma od 126 pb ne smanjuje njegovu rekombinogenost, dok razmaknica duljine 10 pb potpuno stabilizira palindrome duljine 126 i 150 pb.
Abstract (english)
Genomes of all organisms, especially of higher eukaryotes, contain a significant proportion of repeated sequences. A special group of inverted repeats are closely spaced inverted repeats known as palindromes (perfect palindromes) and pseuodopalindromes (imperfect palindromes). A palindrome is composed of two identical inverted repeats which are not separated, whereas inverted repeats in pseudopalindromes are not identical and/or they are separated by one or more base pairs (bp). Palindromes and pseudopalindromes can form DNA secondary structures which can lead to genetic diseases in human. In this study the influence of pseudopalindromes, i.e. the distance between inverted repeats on its recombinogenicity was investigated. As experimental organism yeast Saccharomyces cerevisiae was used. The results of this study showed that the insertion of 4 bp spacer DNA in the center of a 126 bp long palindrome does not reduce its recombinogenicity, but the 10 bp spacer DNA completely abolishes recombinogenicity of 126 and 150 bp long palindromes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:366831
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-08 11:51:04