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master's thesis
Utjecaj UVC zračenja na uzorke biološkog podrijetla
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:171481
Zrnec, Dario
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Zrnec, D. (2012). Utjecaj UVC zračenja na uzorke biološkog podrijetla (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:171481

Zrnec, Dario. "Utjecaj UVC zračenja na uzorke biološkog podrijetla." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:171481

Zrnec, Dario. "Utjecaj UVC zračenja na uzorke biološkog podrijetla." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:171481

Zrnec, D. (2012). 'Utjecaj UVC zračenja na uzorke biološkog podrijetla', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:171481

Zrnec D. Utjecaj UVC zračenja na uzorke biološkog podrijetla [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:171481

D. Zrnec, "Utjecaj UVC zračenja na uzorke biološkog podrijetla", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:171481

Metadata
TitleUtjecaj UVC zračenja na uzorke biološkog podrijetla
Title (english)Effect of UVC radiation on biological samples
AuthorDario Zrnec
MentorVišnja Bačun-Družina (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-07-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Forenzična genetika bavi se utvrđivanjem DNA profila dobivenih DNA analizom tragova biološkog podrijetla. DNA profili identificirani umnažanjem 15 kratkih uzastopno ponavljajućih polimorfnih lokusa (engl. Short Tandem Repeat, STR) i spolnog biljega amelogenina imaju visoku snagu diskriminacije. U forenzičnim laboratorijima, koriste se komercijalna sredstva za čišćenje i UVC lampe za sprječavanje kontaminacije radnih površina prilikom analize uzoraka biološkog podrijetla. UVC zračenje uzrokuje nastanak ciklobutanskih pirimidinskih dimera (CPD), pirimidin 6-4 pirimidon fotoprodukata (6-4 PP), jednolančanih te dvolančanih lomova DNA. Cilj ovog rada bio je ispitati utjecaj UVC zračenja na uzorke i kontrole izolirane DNA te razrijeđene i nerazrijeđene uzorke i kontrole krvi, sline i sperme, kao i odrediti dozu UVC zračenja pri kojoj nije moguće identificirati DNA profil ispitanika, uslijed degradacije DNA.
Abstract (english)
Forensic genetics deals with determining DNA profiles obtained by DNA analysis of traces of biological origin. DNA profiles identified by amplifying 15 Short Tandem Repeats (STR) loci and a sex marker amelogenin have high discrimination power. In forensic laboratories, commercial cleaning agents and UVC lamps are used for the prevention of contamination of work surfaces during the analysis of samples of biological origin. UVC radiation causes formation of cyclobutane pyrimidine dimers (CPD), pyrimidine 6-4 pirimidone photoproducts (6-4 PP) and single and double stranded DNA brakes. The goal of this research is to examine the effects of UVC radiation on isolated DNA samples and controls, disolved as well as non-dissolved samples and control blood, saliva and sperm samples and to determine the dose of UVC radiation at which identification of the DNA profile of the donor is not possible due to DNA degradation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:171481
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-08 12:04:10