prikaz prve stranice dokumenta Unutarstanične interakcije adenovirusnog E3-14.7K proteina s proteinima NF-kappaB signalnog puta
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master's thesis
Unutarstanične interakcije adenovirusnog E3-14.7K proteina s proteinima NF-kappaB signalnog puta
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:476606
Bukal, Vedrana
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bukal, V. (2012). Unutarstanične interakcije adenovirusnog E3-14.7K proteina s proteinima NF-kappaB signalnog puta (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:476606

Bukal, Vedrana. "Unutarstanične interakcije adenovirusnog E3-14.7K proteina s proteinima NF-kappaB signalnog puta." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:476606

Bukal, Vedrana. "Unutarstanične interakcije adenovirusnog E3-14.7K proteina s proteinima NF-kappaB signalnog puta." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:476606

Bukal, V. (2012). 'Unutarstanične interakcije adenovirusnog E3-14.7K proteina s proteinima NF-kappaB signalnog puta', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:476606

Bukal V. Unutarstanične interakcije adenovirusnog E3-14.7K proteina s proteinima NF-kappaB signalnog puta [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:159:476606

V. Bukal, "Unutarstanične interakcije adenovirusnog E3-14.7K proteina s proteinima NF-kappaB signalnog puta", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:476606

Metadata
TitleUnutarstanične interakcije adenovirusnog E3-14.7K proteina s proteinima NF-kappaB signalnog puta
Title (english)Intracellular interactions of Adenoviral E3-14.7K protein with NF-kappaB proteins
AuthorVedrana Bukal
MentorAndreja Ambriović Ristov (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2012-07-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Adenovirusi sadrže mnogo gena koji utječu na imunološki sustav domaćina i suzbijaju njegov odgovor, te se mnogi od tih gena nalaze u ranoj regiji 3 (E3). U ovom radu smo se orijentirali na E3-14.7K imunoregulatorni protein i njegov utjecaj na NF-κB signalni put. NF-κB transkripcijski faktori su glavni regulatori gena zaduženih za imunosni i upalni odgovor, te gena zaduženih za preživljavanje. Ovdje smo pokušali rasvijetliti utjecaj Ad E3-14.7K proteina na NF-κB promotor, utvrditi njegove interakcije s p50 i p65 podjedinicom ovog signalnog puta, kao i s peptidima izvedenim iz E3-14.7K proteina. Nakon transfekcije stanica s E3-14.7K proteinom, zabilježena je aktivacija NF-κB promotora, te je aktivacija bila značajnija nakon tretmana s TNF-α koji je jedan od aktivatora NF-κB signalnog puta. Interakcije između E3-14.7K i p50, te p65 podjedinice su detektirane pomoću BRET tehnike, te je pomoću konfokalnog mikroskopa utvrđeno da E3-14.7K ima tendenciju stupanja u interakcije s p65 podjedinicom.
Abstract (english)
Adenoviruses have a variety of immunoregulatory genes, and many of them are located in early region 3 (E3). In our research we were oriented on E3-14.7K immunoregulatory protein and its influence on NF-κB pathway. NF-κB family of transcription factors are main regulators of immune, inflammatory and cell survival associated genes. We were trying to elucidate influence of Ad E3-14.7K protein on NF-κB promoter, detect its interactions with p50 and p65, as well as for peptides derived from E3-14.7K. Increase in activation of NF-κB promoter was noticed after transfection of cells with E3-14.7K, and this increase was even more significant after the treatment with human TNF – α which is one of the activators of NF-κB pathway. With BRET assay we were able to detect interactions between E3-14.7K and p50, and between E3-14.7K and p65 subunit of NF-κB pathway. E3-14.7K has shown preference to interact with p65, as we confirmed with confocal microscopy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:476606
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-08 12:53:13