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master's thesis
Genotoksični učinak β-adrenergičkog agonista salbutamola na ljudske limfocite, stanice jetre i krvi zamorčadi
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:140716
Ereš, Maja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Ereš, M. (2012). Genotoksični učinak β-adrenergičkog agonista salbutamola na ljudske limfocite, stanice jetre i krvi zamorčadi (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:140716

Ereš, Maja. "Genotoksični učinak β-adrenergičkog agonista salbutamola na ljudske limfocite, stanice jetre i krvi zamorčadi." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:140716

Ereš, Maja. "Genotoksični učinak β-adrenergičkog agonista salbutamola na ljudske limfocite, stanice jetre i krvi zamorčadi." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:140716

Ereš, M. (2012). 'Genotoksični učinak β-adrenergičkog agonista salbutamola na ljudske limfocite, stanice jetre i krvi zamorčadi', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:140716

Ereš M. Genotoksični učinak β-adrenergičkog agonista salbutamola na ljudske limfocite, stanice jetre i krvi zamorčadi [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:140716

M. Ereš, "Genotoksični učinak β-adrenergičkog agonista salbutamola na ljudske limfocite, stanice jetre i krvi zamorčadi", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:140716

Metadata
TitleGenotoksični učinak β-adrenergičkog agonista salbutamola na ljudske limfocite, stanice jetre i krvi zamorčadi
Title (english)Genotoxic effect of β-adrenergic agonist salbutamol on human lymphocytes, liver cells and blood of guinea pigs
AuthorMaja Ereš
MentorKsenija Durgo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Salbutamol je β2-adrenergički agonist koji se u terapeutskim dozama koristi za liječenje kronične opstruktivne bolesti pluća, posebno astme. U dozama 5-10 puta većim od terapeutskih djeluje kao promotor rasta stoke te predstavlja potencijalnu opasnost za zdravlje ljudi i životinja. Cilj ovog rada bio je utvrditi potencijal salbutamola da izazove lomove DNA u tkivu jetre i limfocitima zamorčadi tretiranih salbutamolom (0,25 mg kg-1 i 2,5 mg kg-1 tjelesne mase) te utvrditi njegov genotoksični učinak u rasponu koncentracija od 0,8 µg L-1 do 2,5 mg L-1 na ljudskim limfocitima u in vitro uvjetima uz dodatak metaboličkog aktivatora. Salbutamol u in vivo uvjetima pokazuje antioksidacijsko djelovanje, prvenstveno u stanicama limfocita tretiranih životinja. In vitro salbutamol u koncentraciji od 0,8 µg L-1 djeluje genotoksično.
Abstract (english)
Salbutamol is β2-adrenergic agonist which is used in therapeutic doses for the treatment of chronic obstructive pulmonary diseases, especially asthma. In doses 5-10 times higher than the therapeutic one, it acts as a growth promotor in livestock and as such represents a potential health hazard to humans and animals. In this paper, genotoxic properties of salbutamol were identified; its ability to cause DNA breakage in liver tissue and lymphocytes of guinea pigs (0,25 and 2,5 mg kg-1 of body weight) and DNA damage in human lymphocytes (0,8 to 2,5 mg L-1) with addition of metabolic activator. Salbutamol in vivo reduces DNA damage in the liver and blood cells compared to control, with the apparent reduction in the lymphocytes of treated animals. In vitro, salbutamol at a concentration of 0,8 mg L-1 showed extreme genotoxic effect in comparison to the control.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:140716
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-08 14:00:03