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master's thesis
Karakterizacija transgeničnih topola potencijalno zanimljivih za proizvodnju bioetanola
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:058841
Šećerović, Amra
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Šećerović, A. (2012). Karakterizacija transgeničnih topola potencijalno zanimljivih za proizvodnju bioetanola (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:058841

Šećerović, Amra. "Karakterizacija transgeničnih topola potencijalno zanimljivih za proizvodnju bioetanola." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:058841

Šećerović, Amra. "Karakterizacija transgeničnih topola potencijalno zanimljivih za proizvodnju bioetanola." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:058841

Šećerović, A. (2012). 'Karakterizacija transgeničnih topola potencijalno zanimljivih za proizvodnju bioetanola', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:058841

Šećerović A. Karakterizacija transgeničnih topola potencijalno zanimljivih za proizvodnju bioetanola [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 May 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:058841

A. Šećerović, "Karakterizacija transgeničnih topola potencijalno zanimljivih za proizvodnju bioetanola", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:058841

Metadata
TitleKarakterizacija transgeničnih topola potencijalno zanimljivih za proizvodnju bioetanola
Title (english)Characterization of transgenic poplars potentially interesting for bioethanol production
AuthorAmra Šećerović
MentorBiljana Balen (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2012-07-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj studije, koja je dio europskog EnergyPoplar projekta, je karakterizacija novoproduciranih multitransformanata topole proizvedenih kombinatorijskim pristupom transformacije u kojem su zanimljivi geni biosintetskog puta lignina (CAD, CCR, F5H) i giberlina (GA 20-Ox) kombinirani kako bi potencijalno utjecali na kvalitetu biomase i biljni rast. Uz promijenjeni sadržaj lignina, topola može biti poželjna lignocelulozna sirovina za proizvodnju bioetanola. Novih 14 transgeničnih linija karakterizirano je na molekularnoj i fenotipskoj razini, i uspoređeno sa divljim tipom. Kombinatorijski pristup je rezultirao dobivanjem većine transformanata sa jednim insertiranim tipom gena (79%) i samo 3 transfromanta sa dva i tri insertirana gena (CAD-CCR, CCR-F5H, CAD-CCR-F5H). Potpuna represija ekspresije gena CAD i CCR nije postignuta. Nijedna kombinacija sa GA 20-ox genom nije dobivena. Transgenične linije topole, uzgajane u skoro idealnim uvjetima u stakleniku, nisu pokazale vidljivo zaostajanje u rastu, s iznimkom transformanta sa tri insertirana gena (CAD-CCR-F5H), čiji je rast bio reduciran za oko 40%. Analiza anatomije drva je ukazala na odgođenu lignifikaciju u drvu stabljike većine transfromanta, kao i snažne promjene u intenzitetu autoflourescencije kod dvostrukog transformanta (CCR-F5H). Ove promjene nisu bile povezane niti su utjecale na snažnu modifikaciju kemijskog sadržaja i strukture drva, jer je analiza Near Infrared spektroskopijom ukazala na razlike samo u trostrukom transfromantu (CAD-CCR-F5H). Posljedično, sadržaj lignina u stabljici s uklonjenom korom bio je reduciran samo u navedenom transfromantu.
Abstract (english)
The aim of the study, which is part of the European Energypoplar project, was characterize newly-produced poplar multitransformants, obtained using a combinatorial transformation approach, in which interesting genes of lignin (CAD, CCR, F5H) and gibberellin synthesis pathway (GA 20-Ox) were potentially combined to affect both biomass quality and plant growth. With altered lignin composition, poplar can be a desirable lignocellulosic feedstock for bioethanol production. The 14 new transgenic poplar lines were characterized at the molecular and phenotypic level and compared with wild type. Combinatorial approach resulted in gaining mainly single gene transformants (79%) and only 3 double or triple transformants (CAD-CCR, CCR-F5H, CAD-CCR-F5H). Complete silencing of CAD and CCR genes was not achieved. No combination with GA 20-Ox was obtained. The transgenic lines, grown under the near-optimal growth conditions of the greenhouse, had no apparent growth retardation, except for the triple transformant (CAD-CCR-F5H), whose growth was reduced of 40%. Analysis of wood anatomy indicated a delay and a default in lignification in the stem, for most transgenics; strong changes in autofluorescence intensity were also observed in double transformant (CCR-F5H). This was however not associated to a strong modification of chemical composition and structure of wood, as NIR spectroscopy revealed differences only for the triple (CAD-CCR-F5H) transformant. In addition, lignin content for the debarked stem was only affected in this triple transformant.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:058841
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2017-05-09 07:46:04