prikaz prve stranice dokumenta Disekcija transkripcijsko terminacijskog kompleksa RNA polimeraze II kod kvasca Saccharomyces cerevisiae korištenjem ˝dosage-suppressor assay˝ metode
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master's thesis
Disekcija transkripcijsko terminacijskog kompleksa RNA polimeraze II kod kvasca Saccharomyces cerevisiae korištenjem ˝dosage-suppressor assay˝ metode
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:359833
Mastelić, Angela
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Mastelić, A. (2011). Disekcija transkripcijsko terminacijskog kompleksa RNA polimeraze II kod kvasca Saccharomyces cerevisiae korištenjem ˝dosage-suppressor assay˝ metode (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:359833

Mastelić, Angela. "Disekcija transkripcijsko terminacijskog kompleksa RNA polimeraze II kod kvasca Saccharomyces cerevisiae korištenjem ˝dosage-suppressor assay˝ metode." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:359833

Mastelić, Angela. "Disekcija transkripcijsko terminacijskog kompleksa RNA polimeraze II kod kvasca Saccharomyces cerevisiae korištenjem ˝dosage-suppressor assay˝ metode." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:359833

Mastelić, A. (2011). 'Disekcija transkripcijsko terminacijskog kompleksa RNA polimeraze II kod kvasca Saccharomyces cerevisiae korištenjem ˝dosage-suppressor assay˝ metode', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 03 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:359833

Mastelić A. Disekcija transkripcijsko terminacijskog kompleksa RNA polimeraze II kod kvasca Saccharomyces cerevisiae korištenjem ˝dosage-suppressor assay˝ metode [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2024 December 03] Available at: https://urn.nsk.hr/urn:nbn:hr:159:359833

A. Mastelić, "Disekcija transkripcijsko terminacijskog kompleksa RNA polimeraze II kod kvasca Saccharomyces cerevisiae korištenjem ˝dosage-suppressor assay˝ metode", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:359833

Metadata
TitleDisekcija transkripcijsko terminacijskog kompleksa RNA polimeraze II kod kvasca Saccharomyces cerevisiae korištenjem ˝dosage-suppressor assay˝ metode
Title (english)Dissecting RNA Polymerase II transcription termination complex in Saccharomyces cerevisiae using a dosage-suppressor assay as a tool
AuthorAngela Mastelić
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2011-07-01, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Transkripcija i eksport kompetentnih mRNP-ova su povezani pomoću CTD-a (karboksi terminalne domene) RNA polimeraze II. CTD služi kao platforma za uzastopnu regrutaciju različitih proteinskih faktora. Defektni mRNP-ovi su prepoznati, zadržani i razgrađeni pomoću jezgrinog sustava kontrole kvalitete. Da bi se bolje razumjeli molekularni mehanizmi uključeni u prepoznavanje defektnih mRNP-ova, naš tim je razvio sustav kojim se može poremetiti mRNP biogeneza. NRD kompleks je jedna od glavnih komponenti jezgrinog sustava kontrole kvalitete. Asocijacija NRD-a s CTD-om omogućava prepoznavanje aberantnih transkripata i njihovu eliminaciju pomoću jezgrinog egzosoma. Ovaj način kontrole kvalitete može biti uništen ukoliko su određeni proteini uključeni u terminaciju transkripcije overeksprimirani, te spječavaju prepoznavanje/uništavanje defektnih mRNP-ova. Overeksprimirali smo nekoliko transkripcijsko terminacijskih proteina in vivo i analizirali posljedice u stanicama u kojima su defektni mRNP-ovi bili sintetizirani. Istraživanje je pokazalo da proteini uključeni u terminaciju transkripcije imaju različite uloge: overekspresija nekih od njih može poremetiti proces prepoznavanja defektivnih mRNP-ova, dok drugi ne pokazuju ovakav učinak.
Abstract (english)
Transcription and export of competent mRNPs are coupled by the CTD(Carboxy Terminal Domain) of RNA polymerase II that serves as a platform for sequential recruitment of different protein factors. Defective mRNPs are recognised, retained, and degraded by nuclear quality control. Our team have developed a system to artificially disrupt mRNP biogenesis in the cell to better understand the molecular mechanisms involved in the recognition of defective mRNPs. We have shown that one of the major components of quality control is the NRD complex whose association with the CTD allows the recognition of aberrant transcripts and their elimination by the nuclear exosome. This quality control may be destroyed if certain proteins involved in transcription termination are over-expressed, thus preventing the detection/destruction of defective mRNPs. To understand how this delicate balance between transcription termination proteins and surveillance system operates in the cell, we have over-expressed several transcription termination factors in vivo and analysed the consequences in the cells where defective mRNPs were synthesised. This research has shown that proteins involved in transcription termination have distinct roles: the overexpression of some proteins disrupts the process of recognition of defective mRNPs while the others do not show this effect.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:359833
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-09 09:38:54