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undergraduate thesis
METODE IZOLACIJE DNA ZA LJEKOVITO I AROMATIČNO BILJE
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of agriculture, 2016. urn:nbn:hr:151:803561
Kurilj, Matej
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

Institutional repository: Repository of the Faculty of Agrobiotechnical Sciences Osijek

Cite this document

Kurilj, M. (2016). METODE IZOLACIJE DNA ZA LJEKOVITO I AROMATIČNO BILJE (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:803561

Kurilj, Matej. "METODE IZOLACIJE DNA ZA LJEKOVITO I AROMATIČNO BILJE." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2016. https://urn.nsk.hr/urn:nbn:hr:151:803561

Kurilj, Matej. "METODE IZOLACIJE DNA ZA LJEKOVITO I AROMATIČNO BILJE." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2016. https://urn.nsk.hr/urn:nbn:hr:151:803561

Kurilj, M. (2016). 'METODE IZOLACIJE DNA ZA LJEKOVITO I AROMATIČNO BILJE', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:151:803561

Kurilj M. METODE IZOLACIJE DNA ZA LJEKOVITO I AROMATIČNO BILJE [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2016 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:151:803561

M. Kurilj, "METODE IZOLACIJE DNA ZA LJEKOVITO I AROMATIČNO BILJE", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:151:803561

Metadata
TitleMETODE IZOLACIJE DNA ZA LJEKOVITO I AROMATIČNO BILJE
Title (english)DNA ISOLATION METHODS FOR MEDICINAL AND AROMATIC PLANTS
AuthorMatej Kurilj
MentorSonja Petrović (mentor)
Committee memberSonja Vila (predsjednik povjerenstva)
Committee memberAndrijana Rebekić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2016-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Genetics and Breeding of Plants, Animals and Microorganisms
Abstract
Većinom sve vrste medicinskog, ljekovitog i aromatičnog bilja sadrže velike količine polisaharida, polifenola i ostalih sekundarnih metabolita. Cilj rada bio je opisati postupak izolacije molekule DNA te utvrditi kvalitetu i količinu izolirane DNA kod biljnih vrsta koje se koriste u ljekovite svrhe. Biljne vrste korištene u istraživanju su: ružmarin, kadulja, kamilica, lavanda, ljupčac, menta, ruta i anis. One su uzgajane u kontroliranim uvjetima i nisu bile pod utjecajem vanjske sredine. Prilikom izolacije korištene su tri metode: CTAB, SDS i FA-UniZG-DNA. Tekući dušik korišten je kod homogenzacije biljnog tkiva, dok je kvaliteta i količina izolirane DNA testirana elektroforetski. CTAB metoda se pokazala najuspješnijom za izolaciju DNA iz svih ispitivanih biljnih vrsta.
Abstract (english)
Most of all medical, medicinal and aromatic plants contain large amounts of polysaccharides, polyphenols and other secondary metabolites. Aim of this resarche was to describe the process of isolation of DNA molecules and to determine the quality and quantity of isolated DNA in plant species which are used in medicinal purposes. Plant species used in this research are: rosemary, sage, chamomile, lavender, lovage, mint, anise and routes. They were grown in controlled area and hadn't any impact of extrnal environment. We used used three types of isolation methods: CTAB, SDS and FA-UniZG-DNA. Liquid nitrogen was used in homogenization of plant tissue, while purity and quality of isolated DNA was estimated by electrophoresis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:803561
Study programmeTitle: Agriculture; specializations in: Agroeconomics, Plant production, Mechanization, Zootechnique, Horticulture
Course: Horticulture
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resourceText
File originBorn digital
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Created on2018-01-24 13:35:36