During the storing of wheat the most importatnt thing is to determine optimal conditions for a longer and better quality of storing. One of the most common measures to protect stored products from insects is the use of chemical pesticides. Considering numerous unwanted consequences, long-term and excessive applications of synthetic pesticides, there is a great urge to find new non pesticides measures that would be less harmful to the environment and human health, but at the same time being equaly or more effective against synthetic pesticides. The objective of this research is to determine the insecticidal activity of diatomaceous earth SilicoSec® and formulation based on diatomaceous soil and plant components on three species of pests in laboratory conditions. In research we used adult forms of R. dominica (lesser grain borer), S. oryzae (rice weevil) and T.castaneum (red flour beetle). The wheat that was used is the variety Vulkan, and an inert dust based on diatomaceous earth SilicoSec®, and a formulation obtained from a mixture of DZ Silicosec® and plant components (marked by name F Silico) were tested. Both of this dusts were tested in 4 doses (300, 400, 500 i 600 ppm). Insecticidal activity of DZ SilicoSec® and formulation of F Silico is estimated by mortality of adults after 7 and 14 days and influencing the development posterity of the F1 generation. Results of action of DZ SilicoSec® and formulation of the F Silico on tested pests show different insecticidal activity depending on the dosage and exposure. Among the tested species of storage pests, the most sensitive to the tested dust were T. castaneum and S. oryzae, while R. dominica was more resistant. The results indicate that the natural powder DE SIlicoSec® and the obtained formulation F Silico, can be successfully used for protection of wheat against the main storage pests: R. domenica, S. oryzae, and T. castaneum.