After grain, tobacco is the most commonly cultivated crop in Slatina area. Tobacco production takes place in two phases. The sowing is done in polystyrene containers that are placed in greenhouse with hydroponics in the middle of March. In the middle of May month, the seedlings are moving into a field where continue growth and development. In seedling production, but also in the field, manufacturers are confronted with various diseases that attack this culture. The most significant fungal disease are caused by Alternaria alternata, Botryotinia fuckeliana, Erysiphe cichoracearum, Peronospora hyoscyami, Pythium spp., Sclerotinia sclerotiorum, Thanathephorus cucumeris, Thielaviopsis basicola, Verticillium spp., Phytophthora parasitica. Fungal disease are most commonly treated with fungicides. Tobacco mosaic virus (TMV), tomato spotted wilt virus (TSWV), potato virus Y (PVY), cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), tobacco rattle virus (TRV) are most commonly viruse diseases that attack tobacco. The infected plant needs to be destroyed. The most significant bacteriosis that attack tobacco is caused by the Pseudomonas syringae pv. tabaci. There is a possibility of alleviating symptoms with copper-based agents. In the Slatina area (Gornji Miholjac) in 2018., during the monitoring of the health condition, on seedlings are observed symptoms that cause Pythium species. In the field, the tobacco mosaic virus and the ''wild fire'' bacterium were observed in larger proportions than in previous years. No other major pathogens have been reported.