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master's thesis
Utjecaj zračenja na sterilizaciju hranjive podloge u mikrovalnoj pećnici
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, 2020. urn:nbn:hr:151:656359
Beti, Anamaria
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

Institutional repository: Repository of the Faculty of Agrobiotechnical Sciences Osijek

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Beti, A. (2020). Utjecaj zračenja na sterilizaciju hranjive podloge u mikrovalnoj pećnici (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:656359

Beti, Anamaria. "Utjecaj zračenja na sterilizaciju hranjive podloge u mikrovalnoj pećnici." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:151:656359

Beti, Anamaria. "Utjecaj zračenja na sterilizaciju hranjive podloge u mikrovalnoj pećnici." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:151:656359

Beti, A. (2020). 'Utjecaj zračenja na sterilizaciju hranjive podloge u mikrovalnoj pećnici', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 21 June 2024, https://urn.nsk.hr/urn:nbn:hr:151:656359

Beti A. Utjecaj zračenja na sterilizaciju hranjive podloge u mikrovalnoj pećnici [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2020 [cited 2024 June 21] Available at: https://urn.nsk.hr/urn:nbn:hr:151:656359

A. Beti, "Utjecaj zračenja na sterilizaciju hranjive podloge u mikrovalnoj pećnici", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:151:656359

Metadata
TitleUtjecaj zračenja na sterilizaciju hranjive podloge u mikrovalnoj pećnici
Title (english)Influence of microwave oven's wattage on medium sterilization
AuthorAnamaria Beti
MentorMonika Tkalec Kojić (mentor)
Committee memberTomislav Vinković (predsjednik povjerenstva)
Committee memberMiro Stošić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2020-01-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Vegetable Growing
Abstract
Pri in vitro uzgoju biljaka koristimo hranjive podloge. Hranjive podloge sadrže sve potrebne hranjive elemente potrebne biljci za rast i razvoj. Kako bi biljkama omogućile rast i razvoj u sterilnim uvjetima hranjive podloge moraju se sterilizirati. Najčešće se steriliziraju u autoklavima, no u novije vrijeme moguća je i sterilizacija u mikrovalnoj pećnici koja je jeftinija i dostupnija od autoklava. U istraživanju je korištena mikrovalna pećnica. Istraživanje je obavljeno na dva načina: 1. Prvo istraživanje obavljeno je u trajanju od 5 minuta po tretmanu pri intenzitetu zračenja od 100 W, 180 W, 300 W, 450 W i 600 W. Korišteno je 23 Erlenmayerove tikvice sa 50 mL hranjive podloge. 2. Drugo istraživanje obavljeno je u trajanju od 5 minuta po tretmanu pri intenzitetu zračenja od 180 W, 300 W, 450 W i 600 W. Korišteno je 20 Erlenmayerovih tikvica sa 50 mL hranjive podloge i staklena laboratorijska čaša sa 400 mL hladne destilirane vode. Tijekom istraživanja bilježeno je ključanje hranjive podloge koje je dalo najbolje rezultate za sterilnost pri najvećem intenzitetu zračenja od 600 W. Isto tako kod isparavanja najveći postotak isparavanja imali su tretmani na 600 W, a najmanji na 100 W i 180 W. Prema rezultatima dobivenim u istraživanju može se zaključiti da je potreban veći intenzitet zračenja kako bi došlo do sterilnosti i ključanja hranjive podloge.
Abstract (english)
When growing plants in in vitro conditions, we use nutrient media. Nutrient media contain all the nutrients needed by the plant for growth and development. To allow plants to grow and develop under sterile conditions, the nutrient medium must be sterilized. They are mostly sterilized in autoclaves, but more recently, sterilization in a microwaves is also possible, which is cheaper and more affordable than autoclaves. In this study microwave was used. The search was done in two ways: 1. The first study was performed for 5 minutes per treatment at a radiation intensity of 100 W, 180 W, 300 W, 450 W and 600 W. 23 Erlenmayer flasks were used with 50 mL of nutrient medium. 2. A second study was performed for 5 minutes per treatment at radiation intensity of 180 W, 300 W, 450 W and 600 W. 20 Erlenmayer flasks were used with 50 mL of nutrient medium and a laboratory glass with 400 mL of cold distilled water. During the study, boiling of the nutrient medium was reported, which gave the best results for sterility at the highest radiation intensity of 600 W. During evaporation, treatments at 600 W had the highest percente of evaporation and the treatments at 100 W and 180 W had the lowest percentages. Based on the results of this study it was concluded that a higher intensity of radiation is required for sterility and boiling of the nutrient medium to occur.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:656359
Study programmeTitle: Vegetable and Flower growing
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka povrćarstva i cvjećarstva (magistar/ magistra inženjer/inženjerka povrćarstva i cvjećarstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public notediplomski rad
Created on2020-02-18 14:47:08