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master's thesis
Utjecaj reakcije pH i temperature na razvoj populacije Bradyrhizobium japonicum
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechical Sciences Osijek, 2020. urn:nbn:hr:151:108972
Prtenjača, Krešimir
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

Institutional repository: Repository of the Faculty of Agrobiotechnical Sciences Osijek

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Prtenjača, K. (2020). Utjecaj reakcije pH i temperature na razvoj populacije Bradyrhizobium japonicum (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:108972

Prtenjača, Krešimir. "Utjecaj reakcije pH i temperature na razvoj populacije Bradyrhizobium japonicum." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:151:108972

Prtenjača, Krešimir. "Utjecaj reakcije pH i temperature na razvoj populacije Bradyrhizobium japonicum." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:151:108972

Prtenjača, K. (2020). 'Utjecaj reakcije pH i temperature na razvoj populacije Bradyrhizobium japonicum', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 25 November 2024, https://urn.nsk.hr/urn:nbn:hr:151:108972

Prtenjača K. Utjecaj reakcije pH i temperature na razvoj populacije Bradyrhizobium japonicum [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2020 [cited 2024 November 25] Available at: https://urn.nsk.hr/urn:nbn:hr:151:108972

K. Prtenjača, "Utjecaj reakcije pH i temperature na razvoj populacije Bradyrhizobium japonicum", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:151:108972

Metadata
TitleUtjecaj reakcije pH i temperature na razvoj populacije Bradyrhizobium japonicum
Title (english)The influence of pH and temperature on the development of the Bradyrhizobium japonicum population
AuthorKrešimir Prtenjača
MentorGabriella Kanižai Šarić (mentor)
Committee memberBojan Stipešević (predsjednik povjerenstva)
Committee memberBrigita Popović (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2020-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Agrochemistry
Abstract
Bakterizacija tla i leguminoznih biljaka fiksatorima dušika u današnje je vrijeme neizostavan dio poljoprivredne proizvodnje. Simbiotska interakcija između kvržičnih bakterija i biljke omogućava fiksaciju inače nedostupnog, atmosferskog dušika, što je dovelo do formacija raznih inokulanata kao alternative mineralnim gnojivima koja imaju negativan učinak na tlo. Najveći problem se očituje u preživljavanju sojeva simbiotskih bakterija koje ovise o raznim fizikalno – kemijskim uvjetima okoliša. Isključivi čimbenici za njihov razvoj su vlažnost, temperatura i pH sredine. Svrha ovog istraživačkog rada bila je odrediti vijabilnost stanica Bradyrhizobium japonicum pri različitim temperaturama i prilagođenom pH medija in vitro. Rezultati su pokazali najbolju vijabilnost stanica pri neutralnom pH i pri 5 °C. Dokazana je i hipoteza da će se pri različitoj temperaturi skladištenja i smanjenjem pH proprocionalno smanjiti i broj poraslih kolonija bakterije. Rezultati ukazuju kako su tremperatura skladištenja i pH ključni faktori za preživljavanje rizobija.
Abstract (english)
Bacterization of the soil and leguminous plants with nitrogen fixating bacteria is nowadays an indispensable part of agricultural production. The symbiotic interaction between nodulating bacteria and plants allows the fixation of otherwise inaccessible, atmospheric nitrogen, which has led to the formation of various inoculants as an alternative to mineral fertilizers that have a negative effect on the soil. The issue of utmost importance is the survival of strains of symbiotic bacteria that depend on various physico - chemical conditions of the enviroment. Exclusive factors for their development are humidity, temperature and pH of the environment. The purpose of this study was to determine the viability of Bradyrhizobium japonicum cells at different temperatures and adjusted pH of the medium in vitro. The results showed the best cell viability at neutral pH and at 5 ° C. The hypothesis that the number of grown colonies of the bacterium will proportionally decrease at different storage temperature and decrease in pH has also been proven. The results indicate that storage temperature and pH are key factors for rhizobia survival.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:108972
Study programmeTitle: Ecological Agriculture
Study programme type: university
Study level: graduate
Academic / professional title: magistar/ magistra inženjer/inženjerka ekološke poljoprivrede (magistar/ magistra inženjer/inženjerka ekološke poljoprivrede)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Public noteDiplomski rad
Created on2020-10-01 12:25:45