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undergraduate thesis
Dobivanje presadnica nevena (Calendula officinalis L.) in vitro metodom
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2022. urn:nbn:hr:151:189621
Valenčak, Marta
Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

Institutional repository: Repository of the Faculty of Agrobiotechnical Sciences Osijek

Cite this document

Valenčak, M. (2022). Dobivanje presadnica nevena (Calendula officinalis L.) in vitro metodom (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:189621

Valenčak, Marta. "Dobivanje presadnica nevena (Calendula officinalis L.) in vitro metodom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:151:189621

Valenčak, Marta. "Dobivanje presadnica nevena (Calendula officinalis L.) in vitro metodom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:151:189621

Valenčak, M. (2022). 'Dobivanje presadnica nevena (Calendula officinalis L.) in vitro metodom', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 17 February 2025, https://urn.nsk.hr/urn:nbn:hr:151:189621

Valenčak M. Dobivanje presadnica nevena (Calendula officinalis L.) in vitro metodom [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2022 [cited 2025 February 17] Available at: https://urn.nsk.hr/urn:nbn:hr:151:189621

M. Valenčak, "Dobivanje presadnica nevena (Calendula officinalis L.) in vitro metodom", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:151:189621

Metadata
TitleDobivanje presadnica nevena (Calendula officinalis L.) in vitro metodom
Title (english)Establishment of calendula (Calendula officinalis L.) transplants in vitro
AuthorMarta Valenčak
MentorMonika Tkalec Kojić (mentor)
Committee memberTomislav Vinković (predsjednik povjerenstva)
Committee memberBoris Ravnjak (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
(Department of Plant Production and Biotechnology)
Osijek
Defense date and country2022-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Agronomy
Crop Production
Abstract
Cilj istraţivanja bio je ispitati i utvrditi utjecaj različitih metoda sterilizacije sjemenki zbog odreĎivanja najučinkovitijih postupaka za dobivanje sadnica Calendula officinalis in vitro metodom klijanja sjemenki na hranjivoj podlozi. U Istraţivanju je ispitano djelovanje etanola i varikine u različitim koncentracijama i vremenskim intervalima na dezinfekciju sjemenki nevena. Za pokus korišteno je komercijalno sjeme Calendula officinalis iz Njemačke kupljeno u trgovačkom centru. Pokus se sastojao od 8 tretmana i kontrolnog tretmana. Svaki tretman bio je zastupljen s 5 Erlenmeyer tikvica s hranjivom podlogom na koju se uvodilo po 5 sjemenki. Utjecaj različitih tretmana nije se ispostavio učinkovitim jer se kontaminiralo 44 od 45 hranjivih podloga. Najveća zabiljeţena kontaminacija bila je na kontrolnom tretmanu (sjemenke koje su bile samo u vodi 30 minuta), gdje su sve hranjive podloge bile kontaminirane i s bakterijom i gljivicom. Hranjiva podloga koja se nije kontaminirala sadrţavala je sjemenke koje su bile drţane 30 minuta u otopini varikine u koncentraciji od 10 %.
Abstract (english)
The goal of the research was to examine and determine the impact of different methods of seed sterilization in order to determine the most effective procedures for obtaining Calendula officinalis seedlings in vitro by the method of seed germination on a nutrient medium. In the research, the effect of ethanol and bleach in different concentrations and time intervals on the disinfection of marigold seeds was examined. Commercial Calendula officinalis seeds from Germany bought in a shopping center were used for the experiment. The experiment consisted of 8 treatments and a control treatment. Each treatment was represented by 5 Erlenmeyer flasks with a nutrient medium to which 5 seeds were introduced. The influence of different treatments did not turn out to be effective because 44 out of 45 nutrient media were contaminated. The highest recorded contamination was in the control treatment (seeds that were only in water for 30 minutes), where all nutrient media were contaminated with both bacteria and fungus. The nutrient medium that did not become contaminated contained seeds that were kept for 30 minutes in a 10 % bleach solution.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:151:189621
Study programmeTitle: Agriculture; specializations in: Agroeconomics, Plant production, Mechanization, Zootechnique, Horticulture
Course: Horticulture
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Public notezavršni rad
Created on2022-10-14 10:33:27