Digestija genomske DNA restrikcijskim endonukleazama

Kovač, Jelena

undergraduate thesis

Digestija genomske DNA restrikcijskim endonukleazama

Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2022. urn:nbn:hr:151:726622

Kovač, Jelena

Josip Juraj Strossmayer University of Osijek
Faculty of Agrobiotechnical Sciences Osijek
Department of Plant Production and Biotechnology

Institutional repository: Repository of the Faculty of Agrobiotechnical Sciences Osijek

Cite this document

APA 6th Edition

Kovač, J. (2022). Digestija genomske DNA restrikcijskim endonukleazama (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:151:726622

MLA 8th Edition

Kovač, Jelena. "Digestija genomske DNA restrikcijskim endonukleazama." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:151:726622

Chicago 17th Edition

Harvard

Kovač, J. (2022). 'Digestija genomske DNA restrikcijskim endonukleazama', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, accessed 11 January 2025, https://urn.nsk.hr/urn:nbn:hr:151:726622

Vancouver

Kovač J. Digestija genomske DNA restrikcijskim endonukleazama [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek; 2022 [cited 2025 January 11] Available at: https://urn.nsk.hr/urn:nbn:hr:151:726622

IEEE

J. Kovač, "Digestija genomske DNA restrikcijskim endonukleazama", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Agrobiotechnical Sciences Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:151:726622

Cite this item: https://urn.nsk.hr/urn:nbn:hr:151:726622

Metadata

Title	Digestija genomske DNA restrikcijskim endonukleazama
Title (english)	Restriction endonuclease digestion of genomic DNA
Author	Jelena Kovač
Mentor	Sonja Petrović (mentor)
Committee member	Sonja Vila (predsjednik povjerenstva)
Committee member	Sunčica Kujundžić (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Faculty of Agrobiotechnical Sciences Osijek (Department of Plant Production and Biotechnology) Osijek
Defense date and country	2022-09-29, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Agronomy Genetics and Breeding of Plants, Animals and Microorganisms
Abstract	Restrikcijska endonukleaza je naziv za enzim pronađen u bakterijskim stanicama kao dio njihova restrikcijsko modifikacijskog sustava koji im služi u obrani i razgradnji virusne DNA. Cilj ovog istraživanja je bio izolirati genomsku DNA iz tri biljne vrste s različitom veličinom genoma te provesti uspješnu digestiju genomske DNA restrikcijskim endonukleazama. U radu su korištene vrste Brachypodium distachyon L. i kukruz (Zea mays L.) i šest restrikcijskih endonukleaza. Genomska DNA izolirana je koristeći CTAB metodu, a digestija izolirane DNA je optimizirana te su rezultati cijepanja provjeren elektroforezom. Utvrđeno je da su najpogodniji enzimi za cijepanje genosmke DNA Brachypodium distachyon L., EcoRI i BamHI, a za Zea mays L. to su enzimi HindIII, RspRSII, EcoRI, pri uvjetima inkubacije i volumenu sastavnica restrikcijskih smjesa propisanih od strane proizvođača.
Abstract (english)	Restriction endonuclease is the name for an enzyme found in bacterial cells as part of their restriction modification system that are used in defense and degradation of foreign DNA. The aim of the study was to isolate genomic DNA from three plant species with different genome sizes and to perform successful digestion of genomic DNA with restriction endonucleases. The species Brachypodium distachyon L. and maize (Zea mays L.) and six restriction endonucleases were used in the work. Genomic DNA was isolated using the CTAB method, digestion of the isolated DNA was optimized and the cleavage results were verified by electrophoresis. It was determinedthat the most suitable enzymes for genomic DNA of Brachypodium distachyon L. are EcoRI and BamHI, and for Zea mays L. to su enzimi HindIII, RspRSII, EcoRI, under the conditions of incubation and the volume of components of the restriction mixtures prescribed by the manufacturer.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:151:726622
Study programme	Title: Agriculture; specializations in: Agroeconomics, Plant production, Mechanization, Zootechnique, Horticulture Course: Horticulture Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka agronomije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Public note	završni rad
Created on	2022-10-19 09:29:03

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